用原位核酸杂交法探讨柯萨奇B组病毒与克山病的关系──（黑龙江省急型克山病）

• 作 者: 钟学宽 邹宁 牛美娟 郭彩玲 张凤民 曲章义 周令望 刘阳 赵晓书 杨晓霞 漆炜 刘艺 付桂玲 曾宪惠 谷鸿喜 于维汉
• 机 构: 哈尔滨医科大学克山病研究所，哈尔滨医科大学微生物教研室，哈尔滨医科大学法医教研室，山东地方病防治研究所
• 出 处: 中国地方病学杂志
• 发表日期: 1995年06期
• 关键词: 急型克山病 核酸杂交 萨奇 病毒基因 非病区 肌组织 缺口平移法 杂交信号 意外死亡 地方病防治

为探讨柯萨奇Ｂ组病毒感染与黑龙江省急型克山病的关系。本文用缺口平移法制备生物标记的柯萨奇Ｂ３病毒的ｃＤＮＡ探针与１３例急型克山病尸检心肌组织及脑外伤、ＣＯ中毒等意外死亡的正常成人心肌组织１０例，非病区７～８个月引产胎儿心肌组织１０例的石腊切片进行原位杂交。结果１３例急型克山病中有８例出现阳性杂交信号，即有柯萨奇Ｂ组病毒的ＲＮＡ存在，阳性率达６１．５％。对照组均为阴性，提示黑龙江省急型克山病有柯萨奇Ｂ组病毒感染，可能是急型克山病发病因素之一。

To investigate the relationship between Coxsackie virus B infection and acute Keshan disease in Heilongjiang Province. In this paper, a gap-translation method was used to prepare biomarkers of Coxsackie B3 virus cDNA probe and 13 cases of acute Keshan disease autopsy myocardial tissue and traumatic brain injury, CO poisoning and other accidental death in 10 cases of normal adult myocardial tissue, non-ward 7 10 cases of fetus myocardial tissue in 8 months after birth were paraffin section in situ hybridization. Results In 13 cases of acute Keshan disease, 8 cases showed positive hybridization signal, that is, Coxsackie B virus RNA existed, the positive rate was 61.5%. The control group were negative, suggesting that acute Keshan disease in Heilongjiang Province Coxsackie B virus infection may be one of the pathogenesis of acute Keshan disease.