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目的探讨人参总皂苷(total saponins of panax ginseng,TSPG)抗放射(6.5 Gy)所致骨髓造血细胞损伤衰老的作用。方法 40只雄性C57BL/6小鼠分为4组(每组10只):对照组、放射(irradiation,IR)组、IR+T50(TSPG 50 mg/kg)组、IR+T200(TSPG 200 mg/kg)组。先给药3 d,然后照射,照射后继续给药7 d,检测外周血的变化情况,计数各组骨髓单个核细胞数、骨髓造血细胞集落生成单位(CFU)的数量,观察形态变化;流式细胞术检测周期变化;回收CFU细胞进行β-半乳糖苷酶衰老(SA-β-gal)染色和细胞免疫荧光分析p16Ink4a蛋白的表达水平。结果与IR组比较,给药处理后,IR+T200组的白细胞数增加至(2.19±0.87)(P<0.05),但各组之间红细胞与血小板均无明显变化(P>0.05);IR+T200组的单个核细胞数及集落个数均明显增加(P<0.05);骨髓造血细胞周期G1期细胞比例随药物浓度增加有减少的趋势;SA-β-gal染色和p16Ink4a细胞免疫荧光染色均显示,IR+T200组的CFU中细胞阳性率明显降低(P<0.05)。结论合适剂量的TSPG(200 mg/kg)对放射引起的造血细胞损伤衰老有保护作用。
Objective To investigate the anti-aging effect of total saponins of panax ginseng (TSPG) on bone marrow hematopoietic cells induced by radiation (6.5 Gy). Methods Forty male C57BL / 6 mice were divided into 4 groups (n = 10): control group, IR group, IR + T50 group / kg) group. The rats were sacrificed for 3 d, then irradiated and irradiated for 7 days. The changes of peripheral blood were measured. The numbers of bone marrow mononuclear cells and bone marrow hematopoietic colony forming units (CFU) in each group were counted and the morphological changes were observed. Cytometry was used to detect the cell cycle changes. CFU cells were recovered and the expression of p16Ink4a protein was analyzed by SA-β-gal staining and cell immunofluorescence. Results Compared with IR group, the number of leukocytes in IR + T200 group increased to (2.19 ± 0.87) (P <0.05) after treatment, but there were no significant changes in erythrocytes and platelets between groups (P> 0.05). IR + T200 group, the number of mononuclear cells and the number of colonies were significantly increased (P <0.05); the proportion of cells in G1 phase of bone marrow hematopoietic cells decreased with the increase of drug concentration; the expression of SA-β-gal and p16Ink4a immunofluorescence staining All showed that the positive rate of CFU in IR + T200 group was significantly lower (P <0.05). Conclusion The appropriate dose of TSPG (200 mg / kg) has the protective effect on the hematopoietic cell injury induced by radiation.