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目的探讨ABO正反定型不符的原因及正确定型方法,为安全输血提供质量保障。方法采用全自动血型仪对献血者进行ABO血型鉴定,对正反定型不符或者凝集强度异常的标本,分析其原因,采用不同血型血清学方法进一步试验,进行正确的ABO分型。结果在54例ABO疑难血型标本中,不规则抗体标本26例(48.15%),其中抗-M 15例(27.78%)、抗-P1 7例(12.96%)、抗-H 2例(3.70%)、抗-A1和抗-Leb各1例(1.85%),弱A抗原8例(14.81%),弱B抗原7例(12.96%),弱抗-A 7例(12.96%),弱抗-B 6例(11.11%);最终确定B型22例,A型15例,AB型14例,O型3例。结论当ABO血型鉴定中出现正、反定型不一致时,分析其原因,采用不同的血型血清学方法进行鉴定,正确判定血型,以确保临床输血安全。
Objective To explore the reasons for the discrepancy between positive and negative ABO patterns and to determine the correct methods to provide safe blood transfusion. Methods The ABO blood type was identified by blood type automatic analyzer. The samples with incompatible positive and negative coagulation intensity or abnormal coagulation intensity were analyzed. The reasons were analyzed by different blood group serological methods, and the correct ABO typing was performed. Results Of the 54 ABO blood samples, 26 (48.15%) had irregular antibody samples, of which 15 (27.78%) were anti-M, 7 (12.96%) were anti-P1 and 3.70 ), Anti-A1 and anti-Leb in 1 case (1.85%), weak A antigen in 8 cases (14.81%), weak B antigen in 7 cases (12.96%), weak anti-A (12.96% -B in 6 cases (11.11%). In the final analysis, 22 cases were type B, 15 cases were type A, 14 cases were AB and 3 cases were type O. Conclusion When positive and negative inconsistencies occur in the identification of ABO blood group, the reasons are analyzed, and different blood group serological methods are used to identify and determine the blood group correctly so as to ensure the safety of clinical blood transfusion.