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目的探讨明日叶查尔酮(AC)对2型糖尿病大鼠氧化应激水平的影响。方法雄性Wistar大鼠高脂饲料喂养加链脲佐菌素腹腔注射建立2型糖尿病模型,将造模成功大鼠随机分为糖尿病模型组、AC 30、10、5 mg/kg组及对照组,每日经口灌胃AC连续4周,检测空腹血糖、血清胰岛素、超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力、氧化型低密度脂蛋白(ox-LDL)和胰腺组织病理改变等指标。结果糖尿病模型组大鼠血糖与胰岛素水平分别为(17.30±3.57)mmol/L和(38.28±4.97)mU/L,MDA和ox-LDL含量分别为(12.00±1.32)nmol/mL和(23.05±2.87)ng/mL,均明显高于对照组(P<0.05),与糖尿病模型组比较,AC 30 mg/kg组大鼠血糖[(7.00±2.55)mmol/L]、胰岛素[(29.50±5.31)mU/L]、MDA[(9.96±0.65)nmol/mL]和ox-LDL[(15.36±1.70)ng/mL]均明显降低,差异均有统计学意义(P<0.05),胰岛细胞损伤程度减轻。结论 AC对2型糖尿病大鼠氧化应激和胰岛细胞损伤有一定抑制作用。
Objective To investigate the effect of echinacolone (AC) on oxidative stress in type 2 diabetic rats. Methods Male Wistar rats were fed with high fat diet and streptozotocin intraperitoneally to induce type 2 diabetes mellitus. The successful model rats were randomly divided into diabetic model group, AC 30, 10 and 5 mg / kg group and control group. The rats in each group were given AC for 4 consecutive days. The fasting blood glucose, serum insulin, superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity, ox-LDL, And pancreatic tissue pathology and other indicators. Results The levels of blood glucose and insulin in diabetic rats were (17.30 ± 3.57) mmol / L and (38.28 ± 4.97) mU / L, respectively. The contents of MDA and ox-LDL in diabetic rats were (12.00 ± 1.32) nmol / 2.87) ng / mL were significantly higher than those in the control group (P <0.05). Compared with the diabetic model group, the blood glucose level in the AC 30 mg / kg group was significantly higher than that in the control group [(7.00 ± 2.55) mmol / ) mU / L, MDA 9.96 ± 0.65 nmol / mL and ox-LDL 15.36 ± 1.70 ng / mL, all of which were significantly different (P <0.05) To reduce the extent. Conclusion AC can inhibit oxidative stress and islet cell injury in type 2 diabetic rats.