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用Phenyl—Sepharose 4B柱自牛脑与人脑中提取钙调素,与甲基化牛血清白蛋白混合免疫家兔,获得兔抗钙调素抗体,以其建立了特异性高的ELISA竞争法,定量测定精子钙调素含量。不育男性(n=263)为52.3±20.3ng/10~6精子,生育男性(n=155)为68.7±15.6ng/10~6精子(P<0.01).钙调素含量与精子畸形率、精子活力、精子活率有程度不同的相关关系,相关系数(r)分別为不育组r=-0.988、0.977、0.343;牛育组r=-0.970、0.925、0.010,结果提示,精子功能与结构的异常与钙调素含量有密切关系。
Using Phenyl-Sepharose 4B column to extract calmodulin from bovine brain and human brain and immunizing rabbits with methylated bovine serum albumin to obtain rabbit anti-calmodulin antibody, a specific high ELISA competition method was established , Quantitative determination of sperm calmodulin content. The infertile men (n = 263) were 52.3 ± 20.3ng / 10 ~ 6 spermatozoa and the male fertility (n = 155) was 68.7 ± 15.6ng / 10 ~ , Sperm motility and sperm motility have different degrees of correlation, the correlation coefficients (r) were sterility group r = -0.988,0.977,0.343; oxfed group r = -0.970,0.925,0.010, the results suggest that sperm function And structural abnormalities and calmodulin content are closely related.