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目的了解乙型肝炎(简称乙肝)初筛滴度标本中假阳性所占比率。方法对RPHA检测HBsAg初筛滴度不同的、2006～2008年在乙肝流行病学调查中对规定初筛效价≥1∶4者955份血清的中和试验结果进行分析。结果中和试验阳性率随初筛滴度的升高而增加;初筛效价1∶41,∶8,1∶16及1∶32的血清中和阳性率分别为1.1%,7.1%,76.7%及83.8%;而初筛效价1∶64及其以上血清可100%被抗-HBs中和。结论凡初筛效价在1∶32及其以下的血清必须用中和试验证实其特异性,以排除可能存在的假阳性结果。
Objective To understand the percentage of false positives in hepatitis B (HBV) primary screening titer. Methods The titer of HBsAg was different from that of RPHA. The results of neutralization test of 955 serums with preliminarily screening titer ≥1: 4 in epidemiological survey of hepatitis B from 2006 to 2008 were analyzed. Results The positive rate of neutralization test increased with the increase of the initial screening titer. The positive rates of serum neutralizing antibody of 1:41 ,:8, 1:16 and 1:32 were 1.1%, 7.1%, 76.7 % And 83.8%, respectively; while the primary screening titer 1:64 and above sera can be 100% neutralized by anti-HBs. CONCLUSIONS: Serum that has a primary titer of 1:32 or less must be tested for its specificity using a neutralization assay to exclude possible false-positive results.