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目的观察HBV阳性患者血清中HBV共价闭合环状DNA(cccDNA)的检出率及其与不同疾病状态的关系。方法选取2008年1月-12月收治的慢性HBV感染者120例,其中慢性HBV携带者21例,HBeAg阳性慢性乙型肝炎38例,HBeAg阴性慢性乙型肝炎35例,非活动性HBsAg携带者26例。采用巢式PCR法检测全部患者血清中的HBVcccDNA。结果 120例血清中HBVcccDNA阳性检出率为43.3%(52/120);慢性HBV携带者、HBeAg阳性慢性乙型肝炎、HBeAg阴性慢性乙型肝炎和非活动性HBsAg携带者的cccDNA阳性检出率分别为76.2%(16/21)、63.2%(24/38)、34.3%(12/35)和0%,各组间的差异均有统计学意义(P<0.05);血清高HBVDNA定量组的HBVcccDNA阳性检出率高于低HBVDNA定量组(P<0.05)。结论 HBVcccDNA检出率与外周血HBV复制指标HBVDNA有关,并与不同疾病状态相关。
Objective To observe the detection rate of HBV cccDNA in serum of patients with HBV positive and its relationship with different disease states. Methods A total of 120 patients with chronic HBV infection were enrolled from January 2008 to December 2008, including 21 chronic HBV carriers, 38 HBeAg-positive chronic hepatitis B patients, 35 HBeAg-negative chronic hepatitis B patients and non-active HBsAg carriers 26 cases. Nested PCR was used to detect HBVcccDNA in all patients. Results The positive rate of HBV cccDNA in 120 cases was 43.3% (52/120). The positive rate of cccDNA in chronic HBV carriers, HBeAg-positive chronic hepatitis B, HBeAg-negative chronic hepatitis B and non-active HBsAg carriers (76.2%), 63.2% (24/38), 34.3% (12/35) and 0%, respectively. The differences among the groups were statistically significant (P <0.05) The positive rate of HBVcccDNA was higher than that of low HBVDNA group (P <0.05). Conclusion The detection rate of HBVcccDNA is related to HBVDNA, a marker of HBV replication in peripheral blood and is related to different disease states.