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目的:探讨华西与华北两地戊型肝炎病毒对人群健康影响。方法:采集2个地区的1464份人血清样本采用HEV间接法酶联免疫法(ELISA)检测抗HEV-IgG和HEV-IgM感染指标,对IgM阳性血清进行HEV基因的提取和PCR扩增,进行感染HEV指标检测。结果:华西某市HEV抗体阳性率为45.04%(373/828),华北某市HEV抗体阳性率为27.2%(173/636),P<0.01;其中男性人群中HEV抗体阳性率为40.36%(400/991),女性人群中HEV抗体阳性率为30.87%(146/473),P<0.01。在60岁以下年龄组,华西某市HEV-IgG阳性率高于华北某市,而两地在60岁以上年龄组人群中的HEV-IgG阳性率没有显著性差异。两市人群HEV-IgG阳性率随着年龄的增长而增高,表明男性人群HEV-IgG阳性率高于女性。结论:华西某市人群HEV感染率高于华北某市且感染率男性高于女性。
Objective: To investigate the health effects of hepatitis E virus in West China and North China. Methods: A total of 1464 human serum samples from two regions were collected for detection of HEV-IgM and HEV-IgM infection by HEV indirect enzyme-linked immunosorbent assay (ELISA). HEV gene was extracted and amplified by PCR from IgM positive serum. Infection HEV index test. Results: The positive rate of HEV antibody was 45.04% (373/828) in one city of West China, while the positive rate of HEV antibody was 27.2% (173/636) in one city of North China, P <0.01. The positive rate of HEV antibody in male was 40.36% 400/991). The positive rate of HEV antibody in female population was 30.87% (146/473), P <0.01. In the age group of 60 years old, the positive rate of HEV-IgG in a city in West China was higher than that in a city in North China. There was no significant difference in the positive rate of HEV-IgG between the two groups in the age group of 60 years and over. The positive rate of HEV-IgG in both populations increased with age, indicating that the positive rate of HEV-IgG in male population was higher than that in female. Conclusion: The prevalence of HEV in a population of a city in West China is higher than that of a city in North China and the infection rate is higher in men than in women.