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目的:研究41%草甘膦对小鼠急性中毒和遗传毒性作用。方法:将32只昆明小鼠随机分为4组,雌雄各半,按照霍恩氏法分别采用1000、2150、4640、10000mg/kg草甘膦进行灌胃染毒,染毒量0.2ml/10g,一次性灌胃染毒后观察14天,计数各组动物的死亡数。另外取50只昆明小鼠随机分为5组,雌雄各半,分别设置580、1160、2320mg/kg草甘膦染毒组和阴性对照组、阳性对照组;各染毒组和阴性对照组采用灌胃给药,阳性对照组采用腹腔注射,连续染毒2天,动物于末次染毒6小时后处死,取股骨骨髓涂片,观察小鼠骨髓嗜多染红细胞的微核率的变化。结果:41%草甘膦的小鼠经口LD_(50)为4640mg/kg,无明显性别差异。一定剂量的草甘膦可诱导小鼠骨髓细胞微核率明显上升,且具有剂量依赖关系(r=0.741,P<0.01)。结论:草甘膦对小鼠具有一定遗传毒性作用。41%草甘膦的小鼠经口ID_(50)为4640mg/kg,属低毒农药。
Objective: To study the acute toxicity and genotoxic effects of 41% glyphosate in mice. Methods: 32 Kunming mice were randomly divided into 4 groups, male and female, respectively, according to Horn’s method, respectively, with 1000,2150,4640,10000mg / kg glyphosate gavage, exposure dose 0.2ml / 10g , After a one-time intragastric administration observed for 14 days, the number of animals in each group were counted. Another 50 Kunming mice were randomly divided into 5 groups, male and female, respectively, were set 580,122,12320mg / kg glyphosate exposure group and negative control group, positive control group; each exposure group and negative control group The rats in the positive control group were injected intraperitoneally for 2 consecutive days. The animals were sacrificed 6 hours after the last exposure. The bone marrow of the femur was smeared to observe the change of the micronucleus rate of mouse polychromatic erythrocytes. Results: 41% of glyphosate mice LD_ (50) 4640mg / kg, no significant gender differences. A dose of glyphosate induced a marked increase in the micronuclei of bone marrow cells in a dose-dependent manner (r = 0.741, P <0.01). Conclusion: Glyphosate has a certain genotoxic effect on mice. 41% of glyphosate mice oral ID_ (50) 4640mg / kg, is a low toxicity pesticide.