论文部分内容阅读
目的通过检测外周血CD4+CD25+调节性T淋巴细胞(Treg),了解自身免疫病患儿Treg细胞的变化,以期更好的为临床诊断和治疗提供依据。方法收集2007年11月-2008年6月本院风湿免疫科诊断为自身免疫性疾病患儿93例。本院同期门诊体检健康儿童35例作为健康对照。对发病人数在前3位的类风湿性关节炎(JRA,22例)、SLE(12例)和过敏性紫癜(HSP,12例)分别与健康对照组进行比较。采用流式细胞术检测2组儿童外周血CD4+CD25+T淋巴细胞和CD4+T淋巴细胞,计算CD4+CD25+Treg细胞占CD4+T淋巴细胞百分数。应用SPSS 11.5统计软件对数据进行独立样本t检验。结果1.自身免疫性疾病患儿组CD4+CD25+Treg细胞/CD4+T淋巴细胞、CD4+CD25+T淋巴细胞分别为(21.85±11.68)%、(6.14±3.21)%,健康对照组为(12.83±3.61)%、(3.68±1.02)%,2组比较前者显著高于后者(Pa<0.05)。2.JRA患儿CD4+CD25+Treg细胞/CD4+T淋巴细胞、CD4+CD25+T淋巴细胞分别为(19.49±8.60)%、(6.22±3.11)%,SLE患儿为(31.04±17.38)%、(7.38±4.09)%,HSP患儿为(19.0±9.33)%、(5.80±2.44)%,均显著高于健康对照组(Pa<0.05)。结论自身免疫性疾病患儿CD4+CD25+Treg细胞显著升高,其可能参与了自身免疫性疾病的病理过程。
Objective To detect the changes of Treg cells in children with autoimmune diseases by detecting CD4 + CD25 + regulatory T lymphocytes (Tregs) in peripheral blood in order to provide a better basis for clinical diagnosis and treatment. Methods A total of 93 children with autoimmune diseases diagnosed as rheumatoid arthritis were collected from November 2007 to June 2008 in our hospital. The hospital outpatient check-up of healthy children 35 cases as a healthy control. Rheumatoid arthritis (JRA, 22 cases), SLE (12 cases) and Henoch-Schonlein purpura (HSP, 12 cases) were compared with the healthy control group respectively. Flow cytometry was used to detect CD4 + CD25 + T lymphocytes and CD4 + T lymphocytes in peripheral blood of two groups of children, and the percentage of CD4 + CD25 + Treg cells in CD4 + T lymphocytes was calculated. Data were independently t-tested using SPSS 11.5 statistical software. Results 1. In children with autoimmune diseases, CD4 + CD25 + Treg cells / CD4 + T lymphocytes and CD4 + CD25 + T lymphocytes were (21.85 ± 11.68)% and (6.14 ± 3.21)%, respectively, (12.83 ± 3.61)% and (3.68 ± 1.02)%, respectively. The former two groups were significantly higher than the latter (Pa <0.05). The percentage of CD4 + CD25 + Treg cells / CD4 + T lymphocytes and CD4 + CD25 + T lymphocytes in children with JRA were (19.49 ± 8.60)% and (6.22 ± 3.11)% respectively, and those in SLE children were (31.04 ± 17.38) %, (7.38 ± 4.09)% respectively. The HSP patients were (19.0 ± 9.33)% and (5.80 ± 2.44)%, respectively, which were significantly higher than those in healthy controls (Pa0.05). Conclusion CD4 + CD25 + Treg cells in children with autoimmune diseases are significantly increased, which may be involved in the pathological process of autoimmune diseases.