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Eight new annonaceous acetogenins,squamotin A-D (1-4),annosquatin Ⅳ-Ⅴ (5 and 6),muricin O (7) and squamosten B (8),together with four known ones (9-12) were isolated from the seeds ofAnnona squamosa.Their structures were elucidated by chemical methods and spectral data.The inhibitory activities of compound 1-9 against three multidrug resistance cell lines were evaluated.All tested compounds showed strong cytotoxicity.