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本文介绍了不同百日咳菌株对单层WID细胞(人肠癌类上皮细胞)的粘附能力,证明了丝状血凝素(FHA)在此过程中所起的重要作用。作者将~(35)S蛋氨酸标记的百日咳杆菌与WiDr细胞共同培养后,镜检证明,百日咳To-hama Ⅰ相毒力株可大量粘附在WiDr细胞上.其粘附率与细胞的密度和细菌浓度成正比。Tohama Ⅲ和Ⅳ相无毒力菌株仅有少量粘附,缺乏FHA的突变株粘附也很少,但略多于Ⅲ和Ⅵ相菌株。由Tn5插入的突变株BP353、BP354和自
This article describes the ability of different pertussis strains to adhere to monolayers of WID cells (human colon carcinoid epithelial cells), demonstrating the important role of filamentous hemagglutinin (FHA) in this process. After co-culturing 35G-methionine-labeled Bordetella pertussis with WiDr cells, the adhesion of To-hama Ⅰ to pertussis on WiDr cells was confirmed by microscopic examination.The adhesion rate was correlated with cell density and The concentration of bacteria is proportional. Tohama Ⅲ and Ⅳ non-toxic strains only a small amount of adhesion, lack of FHA mutant strain adhesion is also very small, but slightly more than Ⅲ and Ⅵ phase strains. Mutant strains BP353, BP354 and Tn5 were inserted