用四氧化饿亚铁氰化钾提高PAP染色效果

• 作 者: 刘芳华
• 出 处: 国外医学.临床生物化学与检验学分册
• 发表日期: 1989年02期
• 关键词: 亚铁氰化钾 光镜检查 四氧化锇 染色效果 染色步骤 清晰度 检出 抗原 处理方法 蒸馏水

迄今,PAP 仍是适合光镜检查的最敏感的免疫细胞化学染色方法,但有时却不能保证清晰地检出抗原。作者介绍一种简单易行的处理方法,可提高染色清晰度。先按常规方法进行PAP 染色。PAP 染色步骤完成后再行以下步骤:1.5%四氧化锇作用30分钟;2.蒸馏水反复洗涤;3.20ml 新鲜配制的2%亚铁氰化钾加80ml1%盐酸作用15分钟;

To date, PAP is still the most sensitive immunocytochemical staining method suitable for light microscopy, but sometimes it can not guarantee clear antigen detection. The author introduced a simple and easy way to deal with, can improve the dyed clarity. PAP staining is carried out by conventional methods. PAP dyeing step is completed, the following steps: 1.5% osmium tetroxide for 30 minutes; 2. Repeatedly washed with distilled water; 3.20ml freshly prepared 2% potassium ferrocyanide plus 80ml1% hydrochloric acid for 15 minutes;