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从人精子中提取顶体素，制备兔抗人精子顶体素抗体，建立定量检测顶体素的ELISA方法。本法线性范围为（0．35～3．0）×10－3g／L，批内、批间cV分别为7．8％～9．6％和8．4％～10．8％。临床检测结果表明，在精子密度为50×109／L，生育组（n=15）顶体素含量（X±S）为（1．04±0．10）×10－3g／L；不育组（n=30）顶体素含量为（0．81±0．18）×10－3g／L，两组之间差异显著（P＜0．01），顶体素含量与精子密度、精子顶体完整率之间呈正相关（r=0．987、r=0．821），提示精子顶体素含量的降低是引起男性不育的原因之一。
The apophorin was extracted from human sperm to prepare rabbit anti-human sperm anti-human spermatozoa antibody and the ELISA method was established for the quantitative determination of apocrine. The linear range of this method is (0.35-3.0) × 10-3g / L, and the inter-lot and inter-lot cV are 7.8% -9.6% and 8.4% -10.8% respectively. The results of clinical examination showed that the apocrine contents (X ± S) in the fertility group (n = 15) were (1.04 ± 0.10) × 10-3g / L when the sperm density was 50 × 109 / L; (0.81 ± 0.18) × 10-3g / L, there was significant difference between the two groups (P <0.01). The content of apocrine protein and spermatozoa density, sperm There was a positive correlation between acrosome intact rate (r = 0.987, r = 0.821), suggesting that reduction of apocrine vasoconstriction is one of the causes of male infertility.