论文部分内容阅读
为研究脑脊液（CSF）的正常回流途径，将氚水或HRP作为示踪剂分别缓慢注入两组大折鼠侧脑室。用5μl氘水5～10分钟匀速注入一组大白鼠侧脑室之后，立即处死大白鼠，取出其中部分大白鼠的脉络丛、脑垂体、最后区、松果体等脑室周围器官，经消化、脱色后，加入闪烁液，进行液闪计数。其结果显示上述脑室周围器官放射性活度明显高于本底。取出其余部分大白鼠完整的脑，在但冷切片箱内作正中，偏正中矢状切，切片厚度为3～5μm，于暗室内将上述切片涂布核乳胶，曝光、显影、定影。结果可见脑组织神经层内有散在分布的银颗粒。用20mgHRP溶于0．1ml生理盐水，10～20分钟匀速注入另一组大白鼠侧脑室，约15～30分钟后处死该组大白鼠，取出完整的脑和鼻中隔，行恒冷切片，切片厚30Pm。然后切片孵育，光、电镜观察。于脑室周围器官及脑室周围室管膜层有HRP反应产物的浓集，在鼻中隔的鼻粘膜下组织间隙、嗅丝及其微小静脉周围，以及微小静脉管腔中也可见到HRP反应产物。上述结果提示，CSF可经由脑室周围器官、胸膜及鼻粘膜下静脉网汇入体循环，同时CSF可回流至脑神经层，也参与形成脑神经层组织液，与脑的正常代谢，维持脑的正常功能有关。
To study the normal reflux pathways of cerebrospinal fluid (CSF), tritiated water or HRP as tracer were slowly injected into the lateral ventricle of two rats. Rats were sacrificed immediately after 5 μl of deuterium water was uniformly injected into the lateral ventricle of a group of rats for 5 to 10 minutes and the peripheral organs such as choroid plexus, pituitary gland, final region and pineal gland of some rats were removed and digested and decolorized After adding scintillation fluid, liquid scintillation counting. The results show that the radioactivity around the ventricular organs was significantly higher than the background. Remove the remaining part of the rat brain intact, but in the cold section of the box for the median, partial center of the sagittal section, slice thickness of 3 ~ 5μm, in the dark room will be coated with the above nuclear emulsion, exposure, development, fixation. The results showed that there are scattered scattered silver particles in the brain tissue. 20mgHRP dissolved in 0.1ml saline, 10-20 minutes into another group of rats into the lateral ventricle uniform about 15 to 30 minutes after the rats were sacrificed, remove the intact brain and nasal septum, the line of constant cold section, slice thickness 30Pm. Then slice incubation, light, electron microscopy. HRP reaction products were concentrated in the periventricular organs and periventricular ependymal layer. HRP reaction products were also observed in nasal septum, submucosal tissue space, olfactory filaments and their tiny veins, and in the tiny vein lumen. The above results suggest that CSF can enter the systemic circulation via the periventricular organs, pleura and nasal submucosal venous network, while CSF can be refluxed to the cranial nerve layer, and also participate in the formation of brain nerve layer tissue fluid, and the normal metabolism of the brain to maintain normal brain function related.