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目的 研究次声对下丘的作用机制。方法 用抗 Ｆ Ｏ Ｓ 蛋白和抗神经型一氧化氮合成酶（ｎｃ Ｎ Ｏ Ｓ） 免疫组织化学方法，将大鼠暴露于８ Ｈｚ 、１２０ ｄ Ｂ 的次声中，持续２ 小时，观察下丘 Ｆ Ｏ Ｓ 蛋白的表达情况及其与ｎｃ Ｎ Ｏ Ｓ 阳性神经元的关系。结果 在下丘出现大量 Ｆ Ｏ Ｓ 样及散在的ｎｃ Ｎ Ｏ Ｓ 样免疫反应阳性神经元，且部分ｎｃ Ｎ Ｏ Ｓ阳性神经元的胞核同时呈 Ｆ Ｏ Ｓ 染色阳性，这种 Ｆ Ｏ Ｓ／ｎｃ Ｎ Ｏ Ｓ 双标记神经元约占 Ｆ Ｏ Ｓ 阳性神经元总数的１０ ％ 。结论 部分ｎｃ Ｎ Ｏ Ｓ 神经元参与对次声刺激的反应。
Objective To study the mechanism of infrasound on the inferior colliculus. Methods Immunohistochemical staining with anti-F O S protein and anti-neuronal nitric oxide synthase (ncSOS) was used to expose rats to infrasound at 8 Hz and 120 dB for 2 hours. The expression of F O S protein in the inferior colliculus and its relationship with nc N O S positive neurons. Results A large number of F O S-like and scattered n-c N O S-like immunoreactive neurons were found in the inferior colliculus. Nuclei of some nc N O S positive neurons were positive for F O S staining. This F O S / n C N O S double labeled neurons accounted for about 10% of the total number of F O S positive neurons. Conclusion Part n c N O S neurons involved in the response to infrasound.