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探讨红车轴草提取物(RCE)在体外对小鼠T淋巴细胞和巨噬细胞的影响。MTT法检测RCE对细胞的毒性作用。荧光抗体染色结合流式细胞术检测RCE对T淋巴细胞在Con A的刺激下表达活化抗原CD69、CD25、CD71的影响。CFDA-SE标记技术结合流式细胞术分析RCE对T淋巴细胞在Con A诱导下增殖情况的影响。Griess法检测RCE对小鼠巨噬细胞在LPS刺激24 h后分泌NO的影响。RCE对小鼠有潜在的抗炎作用。RCE对小鼠淋巴细胞和巨噬细胞的细胞毒作用很小。不同质量浓度的RCE能够很好的抑制过量的炎症相关信号分子表达,如NO,CD69,CD25,CD71,且呈剂量依赖性。RCE能够抑制T淋巴细胞的增殖。数据显示RCE可能通过对小鼠淋巴细胞活化与增殖及巨噬细胞NO分泌的抑制展示其抗炎效应。
To investigate the effect of red clover extract (RCE) on mouse T lymphocytes and macrophages in vitro. The cytotoxic effect of RCE on cells was detected by MTT assay. Fluorescent antibody staining and flow cytometry were used to detect the effect of RCE on the expression of activated antigens CD69, CD25, and CD71 by T lymphocytes stimulated by Con A. The effect of RCE on the proliferation of T lymphocytes induced by Con A was analyzed by CFDA-SE labeling technique combined with flow cytometry. The Griess method was used to detect the effect of RCE on the secretion of NO by mouse macrophages after 24 hours of LPS stimulation. RCE has a potential anti-inflammatory effect on mice. The cytotoxic effect of RCE on mouse lymphocytes and macrophages is minimal. Different concentrations of RCE can inhibit the expression of excessive inflammation-related signaling molecules such as NO, CD69, CD25, CD71 in a dose-dependent manner. RCE can inhibit the proliferation of T lymphocytes. The data show that RCE may show its anti-inflammatory effects by inhibiting the activation and proliferation of mouse lymphocytes and the inhibition of NO secretion by macrophages.