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以Fura－2／AM为细胞内钙离子的荧光指示剂,测定谷氨酸（Glu）及过氧化氢（H_2O_2）诱导的新生大鼠脑细胞内游离Ca~2+浓度（［Ca~2+］_i）升高，并观察了神经生长因子（NGF）的影响。结果显示，新生大鼠脑细胞内静息［Ca~2+］_i为208±22nmol·L~-1，NGF对神经细胞静息［Ca~2+］_i无明显影响。NGF1，3，10，30及100μg·L~-1能剂量依赖地抑制Glu和H_2O_2引起的［Ca~2+］_i升高，其IC_50分别为42.5和27.3μg·L~-1。这表明，NGF降低［Ca~2+］_i的水平可能是其保护大脑皮质神经元抗谷氨酸毒性及过氧化氢损伤的机制之一。
Fura-2 / AM was used as a fluorescent indicator of intracellular calcium to measure the intracellular free Ca ~ 2 concentration induced by glutamate (Glu) and hydrogen peroxide (H_2O_2) ] _i) increased, and observed the impact of nerve growth factor (NGF). The results showed that the resting [Ca ~ 2 +] _i in brain cells of newborn rats was 208 ± 22nmol·L ~ (-1), and NGF had no significant effect on resting [Ca ~ 2 +] _i in neurons. NGF 1, 3, 10, 30 and 100 μg · L -1 could inhibit Glu and H_2O_2 -induced [Ca 2+] i increase in a dose-dependent manner with IC 50 of 42.5 and 27.3 μg · L -1, respectively. This suggests that the decrease of [Ca2 +] i by NGF may be one of the mechanisms that protect neurons against glutamate toxicity and hydrogen peroxide damage in cerebral cortex.