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一、怎样制备培养基? 从培养基的配制到培养和繁殖的过程(如图2—1) 本节首先从培养基配制的基本方法到培养前期的简要说明。 1、培养基的计量与混合用于组织培养的培养基,因植物的种类、部位、培养目的不同而各异,但只要选定了最适培养基,那就要备齐所需药品,并用天平分别称量。经常使用的天平有上皿天平和化学天平。称量克以上的药品时用上皿天平。称量100mg(0.1克)以下的药品时用化学天平。在接近最小单位的称量时,误差则变大,
First, how to prepare the medium? From the preparation of the medium to the process of culture and reproduction (Figure 2-1) This section first from the basic method of preparation of the medium to a brief description of pre-culture. 1, the medium of measurement and mixing For tissue culture medium, because of the plant species, location, culture purposes vary, but as long as the selection of the most appropriate medium, it is necessary to prepare the required drugs, and use Balance were weighed. Frequently used balances have a dish scale and a chemical balance. When weighing more than grams of drugs on the dish with balance. Weigh chemical 100mg (0.1g) following the use of chemical balance. In the smallest unit of weighing, the error becomes larger,