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自从1959年Markert等提出了同工酶概念后,在许多学科中得到了广泛的重视和应用。酶带的变化可作为鉴定物种,研究分类、进化、遗传与变异的重要指标。它弥补了以形态结构为基础的经典植物分类法的不足。特别是当形态分类遇到困难时,高分辨率的同工酶技术成为生化分类的主要方法之一。我们从各地收集的多个菌株中,有两个菌株在形态上与其它猴头菌有一定差别,为了得到令人信服的证据,我们用同工酶技术对这两个菌株和其它三个猴头菌的酯酶同工酶、过氧化物酶同工酶、SOD同工酶谱带进行了比较研究,现报道如下:
Since 1959, Markert et al proposed the concept of isozyme, in many disciplines has been widely valued and applied. Changes in the enzyme band can be used as identification of species, research classification, evolution, genetic and variation of important indicators. It makes up for the lack of classical plant taxonomy based on morphological structure. Especially when morphological classification encounters difficulties, high-resolution isoenzyme technology has become one of the main methods of biochemical classification. Two of the strains we collected from all over the world were different in morphology from the rest of Hericium erinaceus. In order to obtain convincing evidence, we used isozyme technology to compare the two strains with the other three monkeys Esterase isozyme, peroxidase isoenzyme, SOD isozyme bands were compared, are reported as follows: