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用体外α-葡萄糖苷酶抑制模型,对槐花乙酸乙酯、石油醚、正丁醇、丙酮水总提取物进行活性筛选,对提取物浓度与抑制活性关系进行了研究,并对强活性的3种浸膏进行了抑制动力学研究。槐花总提取物(IC50=50.56 mg.L-1)、乙酸乙酯部位(IC50=1.25 mg.L-1)、正丁醇部位(IC50=16.14 mg.L-1)。活性远高于对照Acarbose(IC50=1 081.27 mg.L-1);并全部属于非竞争性抑制类型(Ki值分别为4.53、652.5 mg.L-1和62.38mg.L-1);从乙酸乙酯部位得到活性成分槲皮素(IC50=8.86 mg.L-1)和山奈酚(IC50=73.69 mg.L-1)。结果表明,槐花乙酸乙酯部位可作为降血糖活性部位进行体内研究。该文报道的新颖性已为河南大学图书馆2009年6月24日出具的第CX200906242号《科技查新报告》所证实。
The in vitro α-glucosidase inhibition model was used to screen the ethyl acetate, petroleum ether, n-butanol, and acetone water extracts. The relationship between extract concentration and inhibitory activity was studied, and the strong activity was studied. Three kinds of extracts were studied for inhibition kinetics. Total extract of Flos Sophorae (IC50=50.56 mg.L-1), ethyl acetate fraction (IC50=1.25 mg.L-1) and n-butanol fraction (IC50=16.14 mg.L-1). The activity was much higher than that of the control Acarbose (IC50=1081.27 mg.L-1) and all belonged to non-competitive inhibition types (Ki values were 4.53, 652.5 mg.L-1 and 62.38 mg.L-1, respectively); from acetic acid The ethyl ester fraction obtained the active ingredient quercetin (IC50=8.86 mg.L-1) and kaempferol (IC50=73.69 mg.L-1). The results showed that the ethyl acetate part of Achillea can be used as a hypoglycemic active site for in vivo studies. The novelty reported in this article has been confirmed by the “Science and Technology Novelty Report” No. CX200906242 issued on June 24, 2009 by the Henan University Library.