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目的:探讨以土茯苓为主药的中药复方的排铅作用及对小鼠肝组织氧化损伤的保护作用。方法:采用腹腔注射醋酸铅的方法建立铅中毒模型。测定各组小鼠全血及脑、肝脏、肾脏和股骨中铅、锌、铜的含量,并检测肝脏的脂质过氧化水平。结果:中药复方对铅中毒小鼠体质量增长和脏器指数基本无影响。给予中药复方后,铅中毒小鼠血铅、脑铅、肝铅、肾铅和骨铅的含量分别下降了74%、48%、56%、51%和44%,但对体内锌、铜含量影响不显著。中药复方可使铅中毒小鼠肝脏中MDA含量下降31%,同时使GSH含量及GSH-Px、SOD活性分别增高63%、76%和66%。结论:以土茯苓为主药的中药复方不仅具有良好的排铅效果,还能有效拮抗铅中毒小鼠肝组织的氧化损伤。
OBJECTIVE: To investigate the lead excretion effect of Chinese herbal medicine with the main component of Smilax glabra and its protective effect on liver oxidative damage in mice. Methods: Lead poisoning model was established by intraperitoneal injection of lead acetate. The levels of lead, zinc, and copper in whole blood and brain, liver, kidney, and femur were measured in each group of mice, and the level of lipid peroxidation in the liver was measured. Results: Chinese herbal compound had no effect on body weight growth and organ index of lead poisoning mice. After administration of Chinese herbal compound, the levels of blood lead, brain lead, liver lead, kidney lead and bone lead in lead poisoning mice decreased by 74%, 48%, 56%, 51% and 44%, respectively, but zinc and copper contents in the body were reduced. The effect is not significant. Traditional Chinese medicine compound can reduce the liver MDA content in lead poisoning mice by 31%, while increasing GSH content and GSH-Px, SOD activity by 63%, 76% and 66%, respectively. Conclusion: The traditional Chinese medicine compound based on Smilax glabra root not only has good lead expulsion effect, but also can effectively antagonize the oxidative damage of liver tissue in lead poisoning mice.