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Objective:To determine the etiology of acute diarrhea in children under 5 years of age and to improve- knowledge of the etiology of gastrointestinal pathogens using traditional and molecular diagnostic techniques.Methods: Various common enteropathogens(viral,bacterial and parasites) associated with diarrhea were investigated by conventional and molecular techniques(PCR) for verotoxin present in Escherichia coli in 218 children less than 5 years of age admitted to Mansoura University Children hospital- Egypt.Results:The occurrence of enteropathogens identified was as follows:E.coli O157:H7 38.8%followed by Salmonella Spp 29.4%,Aeromonas 20%and Shigella Spp 11.8%.Rotavirus was found in of samples 17.1%.Rotavirus was statistically significant in age<2 yeats old.The commonest parasites found were E.histolytica followed by Enterobius vermicularis, Giardia lambia,Hymenolepis nana and Ascaries.Shigella and Salmonella isolates were tested for their susceptibility to common antimicrobial agents and most of the isolates were resistant to ampicillin and trimethoprim /sulfamethoxazole.Conculsion:This study demonstrated that rotavirus,E.coli O157:H7,Salmonella Spp,and Aeromonas were significant enteropathogens.Rotavirus was significantly associated with infantile gastroenteritis.The results highlight the value of using a combination of traditional and PCR techniques in the diagnosis of enteropathogens related to acute gastroenteritis in children.
Objective: To determine the etiology of acute diarrhea in children under 5 years of age and to improve- knowledge of the etiology of gastrointestinal pathogens using traditional and molecular diagnostic techniques. Methods: Various common enteropathogens (viral, bacterial and parasites) associated with diarrhea were investigated by conventional and molecular techniques (PCR) for verotoxin present in Escherichia coli in 218 children less than 5 years of age admitted to Mansoura University Children hospital-Egypt. Results: The occurrence of enteropathogens identified was as follows: E. coli O157: H7 38.8% followed by Salmonella Spp 29.4%, Aeromonas 20% and Shigella Spp 11.8% .Rotavirus was statistically significant in age <2 yeats old.The commonest parasites found were E.histolytica followed by Enterobius vermicularis , Giardia lambia, Hymenolepis nana and Ascaries. Shigella and Salmonella isolates were tested for their susceptibility to common antimicrobial agents and most of the isolates were resistant to ampicillin and trimethoprim / sulfamethoxazole. Convulsion: This study demonstrated that rotavirus, E. coli O157: H7, Salmonella Spp, and Aeromonas were significant enteropathogens. Rotavirus was significantly associated with infantile gastroenteritis. The results highlight the the value of using a combination of traditional and PCR techniques in the diagnosis of enteropathogens related to acute gastroenteritis in children.