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目的研究瑞舒伐他汀对脑缺血再灌注损伤大鼠白细胞介素(IL)-23和IL-17表达的影响。方法将24只SD大鼠随机分为假手术组、模型组和实验组,每组8只。模型组和实验组按改良Zea-Longa线栓法制备大鼠大脑中动脉栓塞模型,血流阻断1 h后拔出线栓,形成再灌注。假手术组大鼠接受相似手术处理,但是不插入线栓。实验组在模型制作前用瑞舒伐他汀5 mg·kg~(-1)·d~(-1)连续灌胃10 d,qd;假手术组和模型组予以正常喂养。再灌注12 h后,进行神经功能评分,处死大鼠断头取脑,用半定量聚合酶链式反应法和Western blot法分别检测脑组织中IL-23和IL-17的表达水平。结果假手术组无神经功能缺损,IL-23 mRNA和蛋白及IL-17 mRNA和蛋白呈低水平表达。脑缺血再灌注损伤后,与假手术组比较,模型组神经功能评分升高,IL-23 mRNA和蛋白及IL-17mRNA和蛋白表达均明显增高(P<0.05)。与模型组比较,实验组神经功能评分降低,IL-23 mRNA和蛋白及IL-17 mRNA和蛋白表达均明显减少(P<0.05)。结论瑞舒伐他汀可能通过抑制IL-23/IL-17炎症轴发挥脑缺血保护作用。
Objective To investigate the effect of rosuvastatin on the expression of interleukin (IL) -23 and IL-17 in rats with cerebral ischemia-reperfusion injury. Methods Twenty-four SD rats were randomly divided into sham operation group, model group and experimental group, with 8 rats in each group. The middle cerebral artery occlusion model was established by modified Zea-Longa method in the model group and the experimental group. After blocking the blood flow for 1 h, the thrombus was withdrawn to form a reperfusion. Sham-operated rats received similar surgical treatment but did not insert a thread. The experimental group was administrated with rosuvastatin 5 mg · kg -1 · d -1 for 10 days, and the rats in the sham operation group and model group were given normal feeding before model preparation. After reperfusion for 12 hours, the neurological function was evaluated. The rats were sacrificed and the brain was decapitated. The expression of IL-23 and IL-17 in the brain tissues were detected by semi-quantitative polymerase chain reaction and Western blot respectively. Results There was no neurological deficit in sham operation group, and the expression of IL-23 mRNA and protein and IL-17 mRNA and protein were low. After cerebral ischemia-reperfusion injury, the neurological scores of model group were significantly higher than those of sham operation group, and the mRNA and protein expressions of IL-23 and IL-17 mRNA and protein were significantly increased (P <0.05). Compared with the model group, the neurological score decreased and the mRNA and protein expressions of IL-23 and IL-17 mRNA and protein in the experimental group decreased significantly (P <0.05). Conclusion Rosuvastatin may play a protective role in cerebral ischemia by inhibiting the inflammation of IL-23 / IL-17.