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目的研究青木香及复方制剂冠心苏合胶囊中马兜铃酸A在小鼠体内的药动学特点及小鼠在ig给予含相同量马兜铃酸A的青木香和冠心苏合胶囊后,马兜铃酸A的吸收、分布规律的差异。方法采用RP-HPLC法测定血浆中马兜铃酸A的量。色谱条件色谱柱为DiamonsilTMC18柱(250mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(72∶27∶1),体积流量为1.0mL/min,检测波长为315nm,柱温为20℃。结果药动学实验结果显示小鼠分别ig给予青木香和冠心苏合胶囊(相当于2.5mg/kg马兜铃酸A)后,其体内的药动学房室模型均符合一室模型,青木香中马兜铃酸A主要药动学参数t1/2ka、t1/2ke,tmax、AUC、Cmax分别为5.103min、43.63min、17.89min、80.45(μg·min)/mL、0.9168μg/mL;冠心苏合胶囊中相应的参数分别为5.294min、43.50min、18.32min、33.08(μg·min)/mL、0.3818μg/mL。结论小鼠给予含马兜铃酸A相同剂量的青木香和冠心苏合胶囊后,冠心苏合胶囊中马兜铃酸A的Cmax明显低于青木香中的Cmax,说明复方配伍作用可减少马兜铃酸A的吸收。
Objective To study the pharmacokinetics of aristolochic acid A in Qingxinsuhe Capsules in mice and mice and to administer greenwood and Guanxinsuhe capsules containing the same amount of aristolochic acid A in mice. After that, the absorption and distribution of aristolochic acid A were different. Methods The amount of aristolochic acid A in plasma was determined by RP-HPLC. Chromatographic conditions The column was DiamonsilTM C18 column (250mm×4.6mm, 5μm), the mobile phase was methanol-water-glacial acetic acid (72:27∶1), the volume flow rate was 1.0mL/min, the detection wavelength was 315nm, and the column temperature was 20 °C. RESULTS Pharmacokinetics experiments showed that after the mice were given ig to both Aoki and Guanxin Suhe Capsules (equivalent to 2.5 mg/kg of aristolochic acid A), their pharmacokinetic chamber models were all consistent with a one-compartment model. The main pharmacokinetic parameters of aristolochic acid A in A. gondii were t1/2ka, t1/2ke, tmax, AUC, and Cmax were 5.103 min, 43.63 min, 17.89 min, 80.45 (μg·min)/mL, and 0.9168 μg/mL, respectively. The corresponding parameters in Guanxin Suhe capsules were 5.294 min, 43.50 min, 18.32 min, 33.08 (μg·min)/mL, and 0.3818 μg/mL, respectively. Conclusion After mice were given Qingmuxiang and Guanxinsuhe capsules containing the same dose of aristolochic acid A, the Cmax of aristolochic acid A in Guanxinsuhe capsule was significantly lower than that of Cinnamomum amurense, indicating that the compatibility of the compound can be Reduce the absorption of aristolochic acid A.