论文部分内容阅读
本文以晶体结构数据为依据,计算了C-藻蓝蛋白的三聚体和六聚体(天然聚集态)内进行的能量传递过程,结果表明:在荧光发射前,激发能可以在一些途径上重复传递多次,聚集态越高,重复传递次数越少;三聚体中一个单体上的m色团与相邻单体上的f色团有最近的距离和适宜的取向,因而在这样两个色团之间的传递具有最短的时间常数(<1ps),它们构成快速传递对。三聚体内有三个这样的快速传递对,它们主要通过单体内的m(?)f和单体间的f(?)f通道联系,s色团主要与同一单体内的f色团联系;六聚体中,连接两个三聚体的主要通道有两类:即m(?)m通道和s(?)s通道,而f色团上的激发能主要通过同一快速传递对中的m色团传递到另一个三聚体上。这样的六聚体结构具有较高的能量传递效率,成为一种最优结构。
Based on the crystal structure data, the energy transfer process in trimer and hexamer (native aggregate) of C-phycocyanin was calculated. The results show that before the fluorescence emission, the excitation energy can be found in some ways Repeatedly transmitted several times, the higher the aggregation state, the less the number of repeated transmission; Trimer m monomer on a monomer with the neighboring monomer f color group has the closest distance and the appropriate orientation, so in this way The transfer between the two chromophores has the shortest time constant (<1ps), which constitutes the rapid transfer pair. There are three such rapid transfer pairs in the trimer, which are mainly linked through the monomer m (?) F and the monomer f (?) F channel, the s colorimetric mainly with the same monomer f group; There are two main types of mers that connect two trimers: the m (?) M channel and the s (?) S channel, and the excitation energy on the f color group mainly through the same fast pass pair of m colors Mission to pass to another trimer. Such a hexamer structure has a high energy transfer efficiency and becomes an optimal structure.