丝裂原和秋水仙胺对大鼠淋巴细胞粘附于主动脉内皮细胞的影响

• 作 者: 耿永建 茅子均 殷志伟 朱静维
• 机 构: 苏州医学院病理生理学教研窒,苏州医学院病理生理学教研窒,苏州医学院病理生理学教研窒,苏州医学院病理生理学教研窒
• 出 处: 苏州医学院学报
• 发表日期: 1988年03期
• 关键词: 主动脉内皮细胞 粘附 体视学 有丝分裂原 秋水仙胺 大鼠 丝裂原 细胞粘附 主动脉内皮 二维切片

本文用体视学从二维切片估计三维图象的方法,对大鼠淋巴细胞粘附于主动脉内皮细胞的过程进行了初步研究。实验表明,胸腺和肠系膜淋巴结的淋巴细胞中均含有一定数量的粘附细胞。粘附在受淋巴结浸出液刺激的内皮细胞上的细胞数量明显增多。有丝分裂原ConA、PWM和LPS在不同程度上能够促进胸腺或肠系膜淋巴结细胞对主动脉内皮细胞的粘附,其中ConA和PWM作用较强。一定剂量的秋水仙胺可部分抑制肠系膜淋巴结细胞对主动脉内皮细胞的粘附。

In this study, stereological methods were used to estimate three-dimensional images from two-dimensional slices, and the adhesion of rat lymphocytes to aortic endothelial cells was preliminarily studied. Experiments show that lymphocytes in the thymus and mesenteric lymph nodes contain a certain amount of adherent cells. The number of cells adherent to endothelial cells stimulated with lymph leachate increased significantly. The mitogen ConA, PWM and LPS can promote the adhesion of thymus or mesenteric lymph node cells to aortic endothelial cells to varying degrees, of which ConA and PWM are more effective. A certain dose of colchicine partially inhibits the adhesion of mesenteric lymph node cells to aortic endothelial cells.