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目的:检测细胞角蛋白(CK)在结肠癌中的表达,探讨其在病理分级及临床分期中的表达差异性。方法:采用免疫组化法、免疫细胞化学法和Western blot检测结肠癌中CK7和CK19的表达,分析这些表达与肿瘤分期及分型的关系。结果:CK7和CK19在正常结肠组织中均不表达。CK7在结肠癌组织中不表达,而CK19在结肠癌组织中阳性表达。两组间检验差异性明显(P<0.05)。结论:CK7和CK19与结肠癌的发生有关,CK7-/CK19+可作为结肠癌的表达特征之一。
Objective: To detect the expression of cytokeratin (CK) in colon cancer and to explore its difference in the pathological grade and clinical stage. Methods: The expressions of CK7 and CK19 in colorectal carcinoma were detected by immunohistochemical method, immunocytochemistry and Western blot. The relationship between these expressions and tumor staging and typing was analyzed. Results: CK7 and CK19 were not expressed in normal colon tissues. CK7 is not expressed in colon cancer tissues, while CK19 is positively expressed in colon cancer tissues. The difference between the two groups was significant (P <0.05). Conclusion: CK7 and CK19 are associated with the development of colon cancer. CK7- / CK19 + may be one of the features of colon cancer.