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以巴西橡胶种子为外植体；以浓度为９μｍ的３，４—二氯苯氧乙酸（３，４—Ｄ）、苄基氨基嘌呤（ＢＡＰ）培养基为对照，与浓度为４．５、２．２５、０．４５μｍ的培养基作诱导愈伤组织发生和胚胎发生能力的比较。与对照培养基相比，在培养的最初２０天内，浓度为４．５μｍ的培养基可以提高胚胎发生的潜力（ＥＬＨａｄｒａｍｉ等，１９９１，ＡｎｎａｌｓｏｆＢｏｔａｎｙ６７、５１１—５１５），并使腐胺、亚精胺及精胺在培养期间（第４０－７０天）保持在一个较高的水平上，同时使过氧化物酶活性维持在一个低的水平上。在对照培养基中，愈伤组织胚胎发生不良可以认为是组织迅速老化的结果，它具有如下特点：①提早和加速能促进胚胎发生能力的多胺类物质的暂时性的产生；②提早出现过氧化物酶活性的高峰，这与愈伤组织褐化呈正相关，而褐化是胚胎发生的限制因素之一。
The seeds of Brassica campestris were used as explants. The concentrations of 3,4-dichlorophenoxyacetic acid (3,4-D) and benzylaminopurine (BAP) 2.25,0.45μm medium for callus induction and embryogenesis ability comparison. Media with a concentration of 4.5 [mu] m can increase the potential for embryogenesis compared to control media (EL Hadrami et al., 1991, Annals of Botany 67, 511-515) within the first 20 days of culture and make putrescine, spermidine and Spermine remained at a high level during culture (days 40-70) while maintaining peroxidase activity at a low level. In the control medium, callus embryo dysplasia can be considered as a result of rapid tissue aging, which has the following characteristics: ① early and accelerate the embryo can promote the ability of polyamines temporary production; ② early appeared The peak of oxidase activity was positively correlated with callus browning, and browning was one of the limiting factors in embryogenesis.