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目的基因和目标性状遗传稳定性是转基因植物新品种选育和产业化的重要前提。以进入农业部环境释放的抗虫抗除草剂双价转基因玉米Hi II-NGc-1为试验材料,采用PCR、RT-PCR和免疫试纸条技术检测转基因玉米连续3代目的基因cry NGc和bar整合及表达的遗传稳定性,采用心叶期除草剂草铵膦抗性鉴定和心叶期/穗期亚洲玉米螟抗性鉴定实验详细分析了目标性状的遗传稳定性。研究结果表明:Cry NGc和PAT蛋白在Hi II-NGc-1中连续3代遗传稳定,高世代稳定耐受正常5倍大田除草剂草铵膦使用浓度,心叶期和穗期高抗亚洲玉米螟性状稳定,具有潜在的产业化应用价值。转基因玉米Hi II-NGc-1目的基因和目标性状遗传稳定性的明确为开展下一步生物安全评价奠定了基础,对复合性状转基因抗虫抗除草剂玉米新品种的选育和产业化具有重要意义。
The genetic stability of the target gene and the target trait is an important prerequisite for the breeding and industrialization of new varieties of transgenic plants. The bivalent transgenic corn Hi II-NGc-1 released from the Ministry of Agriculture was used as test material to detect cry NGc and bar genes of transgenic maize for three consecutive generations by PCR, RT-PCR and immunoblotting techniques Integration and expression of genetic stability, the use of leaf-stage herbicide glufosinate resistance identification and leaf stage / spike resistance of Asian corn borer experiments to analyze the genetic stability of the target trait. The results showed that the Cry NGc and PAT proteins were inherited in Hi II-NGc-1 for three consecutive generations and were stable in the high-generation for five times of normal. The field herbicide glufosinate use concentration, leaf stage and heading stage were highly resistant to Asian corn Stem borer stable, with potential industrial application value. The clear genetic stability of target gene and target trait of transgenic maize Hi-NGc-1 lays the foundation for the next biosecurity evaluation and plays an important role in the breeding and industrialization of new varieties of transgenic insect-resistant and herbicide-resistant maize .