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目的探讨p16蛋白和增殖细胞核抗原(PCNA)在鳞状细胞癌组织中的表达及其与临床病理特征的关系。方法利用免疫组织化学法(IHC)检测50例鳞状细胞癌患者的癌组织、癌旁组织和30例健康人群的正常组织中p16蛋白和PCNA的表达情况,并分析p16蛋白和PCNA与鳞状细胞癌临床病理特征的关系。结果鳞状细胞癌组织中p16蛋白阳性表达率显著低于癌旁组织和正常组织(P<0.01),鳞状细胞癌组织中PCNA指数显著高于癌旁组织和正常组织(P<0.01);p16蛋白阳性表达率随着癌组织浸润深度增加而降低(P<0.01),PCNA指数随着癌组织浸润深度增加而增大(P<0.01);PTNMⅠ~Ⅱ期p16蛋白阳性表达率显著高于PTNMⅢ~Ⅳ期(P<0.01),PTNMⅠ~Ⅱ期PCNA指数显著低于PTNMⅢ~Ⅳ期(P<0.01);p16阳性表达的鳞状细胞癌组织中PCNA指数为30.67±9.82,显著低于p16阴性表达的鳞状细胞癌组织中的51.96±12.43(P<0.01)。结论在鳞状细胞癌组织中p16蛋白缺失,而PCNA大量表达,且p16蛋白和PCNA表达与鳞状细胞癌的病理分期和浸润深度密切相关,提示p16蛋白和PCNA可作为评价鳞状细胞癌病变程度的重要指标,对指导治疗鳞状细胞癌有重大意义。
Objective To investigate the expression of p16 protein and proliferating cell nuclear antigen (PCNA) in squamous cell carcinoma and its relationship with clinicopathological features. Methods Immunohistochemistry (IHC) was used to detect the expression of p16 protein and PCNA in normal tissues of 50 cases of squamous cell carcinoma, adjacent tissues and 30 healthy persons. The expressions of p16 protein and PCNA were compared with squamous Relationship between clinicopathological features and cell carcinoma. Results The positive rate of p16 protein in squamous cell carcinoma was significantly lower than that in paracancerous tissues and normal tissues (P <0.01). The PCNA index in squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues and normal tissues (P <0.01). The positive expression rate of p16 protein decreased with the depth of invasion (P <0.01), while the PCNA index increased with the depth of invasion (P <0.01). The positive expression rate of p16 protein in PTNM Ⅰ ~ Ⅱ was significantly higher than that The PCNA index of PTNM Ⅰ ~ Ⅱ phase was significantly lower than that of PTNM Ⅲ ~ Ⅳ phase (P <0.01), and the PCNA index of p16 positive squamous cell carcinoma was 30.67 ± 9.82, which was significantly lower than that of p16 Negatively expressed squamous cell carcinoma tissues were 51.96 ± 12.43 (P <0.01). Conclusions The expression of p16 protein and PCNA is significantly decreased in squamous cell carcinoma, and the expression of p16 protein and PCNA is closely related to the pathological stage and depth of invasion of squamous cell carcinoma, suggesting that p16 protein and PCNA can be used as a marker of squamous cell carcinoma Degree of an important indicator of the guiding treatment of squamous cell carcinoma is of great significance.