论文部分内容阅读
以抗旱性差异较大的亲本小白粳子和空育131以及其后代180个F2∶3家系群体为试验材料,构建了包含99个SSR分子标记的遗传连锁图谱,利用浓度为15%PEG-6000模拟干旱胁迫条件。在两种条件下,共检测到影响胚芽鞘长、苗高、主根长和根数的QTL 26个,分别位于水稻的第1、2、3、4、5、6、7、8、12条染色体上,贡献率变幅在4.53%～37.22%之间。其中控制苗高的QTL11个,控制胚芽鞘长的QTL 5个,控制主根长的QTL 5个,控制根数的QTL 5个。在第2条染色体的RM1358-RM1347区间和第6条染色体上的RM461-RM162区间发现了控制多个性状的QTL,在第2条染色体的RM1358-RM1347区间和第8条染色体的RM1384-RM547区间还检测到了在两种条件下同时控制胚芽鞘长和主根长的QTL。
The genetic linkage map containing 99 SSR markers was constructed with the parents of Xiaoyongjing and Kongyu 131 and 180 F2: 3 pedigree populations with large differences in drought resistance. Using 15% PEG- 6000 simulated drought stress conditions. Under the two conditions, a total of 26 QTLs affecting the length, seedling height, main root length and root number of the coleoptile were detected, which were located in the 1st, 2nd, 3rd, 4th, 5th, 6th, On the chromosome, the contribution rate varied between 4.53% ~ 37.22%. Among them, 11 were QTL controlling seedling height, 5 were QTL controlling sheath length, 5 QTL controlling main root length and 5 QTL controlling root number. A QTL controlling multiple traits was found between the RM1358-RM1347 interval on chromosome 2 and the RM461-RM162 interval on chromosome 6, ranging from RM1358 to RM1347 on chromosome 2 and RM1384 to RM547 on chromosome 8 QTLs were also detected that controlled both coleoptile length and root length under both conditions.