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AIM Tot examinet thet rolet oft micro RNAt 1181t(miR-1181)t intinvasiontandtproliferationtintpancreatictcancer.METHODS We analyzedt thet expressiont oft mi R-1181t int severaltpancreatict cancert cellt linest andt generatedt stabletMIA-Pa Ca-2t andt PANC-1t cellt linest witht up-regulatedtmi R-1181t expressiont usingt ant adenovirust deliverytsystem.t Wet thent investigatedt mi R-1181’st effectt ontinvasiont andt proliferationt oft pancreatict cancert cellst byttranswellt assay,t woundt healingt assay,t cellt countingtkit-8t assayt andt colony-formingt assay,t andt exploredtanyt underlyingt mechanismst byt westernt bolt.t Beyondtthat,t wet observedt thet changet oft thet PANC-1t cell’stcytoskeletontbytimmunofluorescencetstaining.RESULTS Ourt datat showedt thatt mi R-1181t wast relativelyt downregulatedtintpancreatictcancertcelltlinestcomparedtwithtnormaltpancreatictductaltepithelialtcells.tAndtmiR-1181tinhibitedt thet migration,t invasiont andt proliferationtactivitiest oft MIA-Pa Ca-2t andt PANC-1t cells.t Notably,aftert over-expressingt oft mi R-1181t int PANC-1t cells,tF-actint depolymerized.t Immunofluorescencet stainingtshowst decreasedt F-actint andt β-tubulint expressiont intPANC-1t cellst over-expressingt mi R-1181t comparedtwitht thet controlt cells.t Furthermore,t wet foundt thattover-expressingt mi R-1181t inhibitedt thet expressiontoft signalt transducert andt activatort oft transcriptiont 3(STAT3)t whilet knocking-downt mi R-1181t up-regulatedtthet expressiont oft STAT3.t Knocking-downt mi R-1181tpromotedt thet invasiont andt proliferationt oft pancreatictcancert cells.t Andt inhibitiont oft STAT3t blockedt thetpromotiont effectst oft knocking-downt mi R-1181t ontproliferationtandtinvasiontintpancreatictcancer.tCONCLUSION Togethert ourt findingst suggestt thatt mi R-1181t maytbet involvedt int pancreatict cancert cellt invasiont andtproliferationt byt targetingt STAT3t andt indicatet thattmi R-1181t mayt bet at potentialt therapeutict agentt fortpancreatictcancer.
AIM To t the t role t of micro RNA 1181 (miR-1181) tin tinvasion tand tproliferation tin tpancreatic tcancer.METHODS We analyzed t the t expression t mi R-1181 t in tpancreatic t cancer t cell t lines t generated t stable tMIA-Pa Ca-2 t and t PANC-1 t cell t lines t with up-regulated tmi R-1181 t expression t an adenovirus delivery tsystem. T We t then t investigated t mi R-1181’s t effect on tinvasion t propagation t of t pancreatic cancer t cells t by ttranswell t assay, t wound t healing t assay, t cell t counting tkit-8 t assay t and colony-forming t assay, t explored tany underlying t mechanisms t by t western t bolt. t Beyond tthat, t we t observed t the t change t of t the PANC-1 t cell tcytoskeleton tby timmunofluorescence tstaining.RESULTS Our t data that t mi R-1181 t was t downregulated tin tpancreatic tcancer tcell tlines tcompared twith tnormal tpancreatic tductal tepitheli al tcells. tAnd tmiR-1181 tinhibited t the migration, t invasion t and population proliferation tactivities t of MIA-Pa Ca- 2 t and PANC- 1 t cells. t Notably, after t over-expressing t of r mi R-1181 t in PANC-1 t cells, tF-actin t depolymerized. t Immunofluorescence t staining tshows t decreased t F-actin t and β-tubulin t expression t in PANC-1 t cells t over-expressing t mi R-1181 tw twith t t control t cells. t Furthermore, t we t found t that tover-expressing t mi R-1181 t suppressed t the t expression tof t signal t transducer t and t activator t of t transcription t 3 (STAT3) t while knocking-down t mi R-1181 t up-regulated t t t of t STAT3. t Knocking-down t mi R-1181 tpromoted t invasion t and t infection t of pancreatic tcancer t cells. t And t inhibition t of STAT3 t blocked t the tpromotion t effects t of knocking-down t mi R-1181 t on tproliferation tand tinvasion tin tpancreatic tcancer. tCONCLUSION Together t our t findings t su ggest t that t mi R-1181 t may tbe t involved t in pancreatic cancer t cell t invasion t and tproliferation t by t targeting t STAT3 t and t include tmi R-1181 t may t be ta t potential t therapeutic t agent t for tpancreatic tcancer.