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纯化从发酵培养液和其它天然来源的生物分子 ,分离步骤较多 ,会造成一定的损失。通过待分离的“靶”分子与载体分子反应 ,形成复合物 ,可选择性地纯化“靶”分子。反应形成的复合物由于在有机相中的溶解性提高 ,从而使“靶”分子从复杂的培养液混合物中分离出来的方法称为“反应萃取”。该方法克服了常规分离技术的许多缺点 ,在降低分离过程中产品的损失方面很有潜力。本文报道了该方法在一些药物 ,包括氨基酸和二肽分离中的应用。实验系统是一套允许两种溶液分别流过管层和壳层的 ,具有列管式结构的中空纤维膜组件。料液和有机溶剂分别流过管层和壳层。靶溶质分子扩散到水 -有机相界面与载体形成复合物 ;载体-溶质复合物被转运到壳层而被重新回收得到。该项研究的目的有以下三方面 :1.应用商品化的膜组件 ,研究在不同条件 (如料液的 p H和有机相浓度 )下 ,溶质的萃取百分率 ;2 .直接应用实验数据推导出模式方程以确定总传质系数 ;3.尝试从“工业的”样品中分离掉杂质 (如多肽 )。
Purification from fermentation broth and other natural sources of biomolecules, the separation step more, will cause some loss. The “target” molecule can be selectively purified by reaction of the “target” molecule to be separated with the carrier molecule to form a complex. The complex formed by the reaction is referred to as “reactive extraction” due to increased solubility in the organic phase, thereby separating “target” molecules from the complex mixture of broths. This method overcomes many of the shortcomings of conventional separation techniques and has great potential in reducing the loss of product during the separation process. This paper reports the application of this method in the separation of some drugs, including amino acids and dipeptides. The experimental system is a hollow fiber membrane module with a tube-and-tube structure that allows both solutions to flow through the tube and shell, respectively. Feed and organic solvent flow through the tube and shell, respectively. The target solute molecules diffuse into the water-organic interface to form a complex with the carrier; the carrier-solute complex is transported to the shell to be recovered again. The purpose of this study is to study the following three aspects: 1. Using commercially available membrane modules, to study the percent of solute extraction under different conditions (such as p H and organic phase concentrations of the feed solution); 2. Deriving directly from the experimental data Model equations to determine the total mass transfer coefficient; 3. Attempts to separate impurities (such as polypeptides) from “industrial” samples.