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目的探究贝伐珠单抗对结直肠癌细胞串珠素表达的影响。方法将处于对数生长期的肿瘤细胞(HCT116和HT29细胞)分为两组:对照组和实验组(加入终浓度为400μg/L的贝伐珠单抗48小时),采用RTPCR检测结直肠癌细胞串珠素mRNA表达。将同一批处于对数生长期的肿瘤细胞分为6组,一组为对照组,其余5组实验组分别加入终浓度为25、50、100、200和400μg/L的贝伐珠单抗,48小时后ELISA检测直肠癌细胞及培养液上清的串珠素蛋白水平,Western blot检测结直肠癌细胞串珠素的蛋白表达。结果与对照组比较,实验组肿瘤细胞串珠素mRNA及蛋白水平均上调(均P<0.05)。在细胞培养液中,与对照组比较,实验组的串珠素蛋白水平未出现显著变化(P>0.05)。结论贝伐珠单抗引起结直肠癌细胞串珠素mRNA和蛋白表达的上调,但未引起细胞培养液中串珠素蛋白的显著变化。
Objective To investigate the effect of bevacizumab on the expression of beads in colorectal cancer cells. Methods The tumor cells in logarithmic growth phase (HCT116 and HT29 cells) were divided into two groups: the control group and the experimental group (48 hours of addition of bevacizumab at a final concentration of 400 μg / L), and the detection of colorectal cancer by RTPCR Cyclosporin mRNA expression. The same batch of tumor cells in logarithmic growth phase were divided into 6 groups, one group as the control group, and the remaining 5 groups were added to the final concentration of 25, 50,100,200 and 400μg / L of bevacizumab, The levels of beads beads in rectal cancer cells and culture supernatants were detected by ELISA 48 hours later. The expression of beads beads in colorectal cancer cells was detected by Western blot. Results Compared with the control group, the mRNA and protein levels of perlecan in the experimental group were up-regulated (all P <0.05). In the cell culture medium, compared with the control group, there was no significant change in the levels of beads beads in the experimental group (P> 0.05). Conclusion Bevacizumab induces the up-regulation of periostin mRNA and protein expression in colorectal cancer cells, but not significant changes of perlecan protein in cell culture medium.