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用重组人γ－干扰素（ＩＦＮ）和肿瘤坏死因子（ＴＮＦ）体外净化骨髓中残存的白血病细胞时，１０００Ｕ／ｍｌＩＦＮ或１００Ｕ／ｍｌＴＮＦ单独应用时仅能清除大约８０％左右，而二者联用时则能起到协同抗白血病的效应，可清除９０％以上残存的白血病细胞，但对ＣＦＵ－ＧＭ的形成却没有协同抑制作用，抑制率仅为５４％。在高热体外净化研究中，单独加热到４１℃作用２ｈ，清除率达９０％，若同时加入１０００Ｕ／ｍｌ的ＩＦＮ，可大大增强高热的净化效果，清除率可达９８％以上，且减轻了高热对ＣＦＵ－ＧＭ的抑制作用，ＣＦＵ－ＧＭ的抑制率下降为２４．５％，与单纯高热净化时的３２．８％相比，Ｐ＜０．０５。因此可以利用提高温度的办法来进一步达到体外净化的目的
In vitro purification of residual leukemia cells in the bone marrow using recombinant human interferon-gamma (IFN) and tumor necrosis factor (TNF), only approximately 80% of 1000 U/ml IFN or 100 U/ml TNF can be eliminated when used alone, and when they are used together The synergistic anti-leukemia effect can eliminate more than 90% of the remaining leukemia cells, but there is no synergistic inhibition effect on the formation of CFU-GM, inhibition rate is only 54%. In the high-calorie in-vitro purification study, heating alone to 41°C for 2 hours resulted in a clearance rate of 90%. If 1000U/ml IFN is added at the same time, the high-temperature purification effect can be greatly enhanced, the clearance rate can reach over 98%, and the high fever can be reduced. For the inhibitory effect of CFU-GM, the inhibitory rate of CFU-GM decreased to 24.5%, compared with 32.8% for pure hyperthermia, P<0.05. Therefore, the method of raising the temperature can be used to further achieve the purpose of in vitro purification.