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为探讨一氧化氮 (NO)的前体左旋精氨酸 (L -arg)对急性心肌梗塞 (AMI)大鼠心肌有无保护作用及其作用机制 ,采用垂体后叶素 (Pit)AMI模型。设立正常对照组、阳性对照组、及L -arg治疗组 ,观察中性粒细胞 (PMN)L -选择素及心肌微血管内皮细胞P -选择素的表达 ,并测定心肌梗塞范围、PMN浸润数及血浆NO、丙二醛 (MDA)的浓度。结果表明 :阳性对照组L -选择素及P-选择素表达上调 ,梗塞心肌PMN浸润数、血浆MDA的浓度明显升高 ,血浆NO的浓度降低。L-arg治疗组上述指标改变的程度明显减轻 (P <0 0 1) ,心肌梗塞范围缩小。提示外源性L -arg对AMI有防治意义 ,其作用机制与NO抑制选择素的表达有关。
To investigate the protective effect of L-arginine (L-arginine), a precursor of nitric oxide (NO) on myocardium in acute myocardial infarction (AMI) rats and its mechanism, a pit AMI model was used. The normal control group, positive control group and L - arg treatment group were established. The expression of L - selectin and P - selectin in myocardial microvascular endothelial cells were observed. The extent of myocardial infarction, the number of PMN infiltration, Plasma NO, malondialdehyde (MDA) concentration. The results showed that in the positive control group, the expression of L - selectin and P - selectin were up - regulated, the infiltration of PMN in infracted myocardium and the concentration of plasma MDA were significantly increased while the concentration of NO in plasma was decreased. L-arg treatment group significantly reduced the extent of the above indicators (P <0.01), myocardial infarction narrowed. It is suggested that exogenous L-arginine can prevent and treat AMI, and the mechanism of action is related to NO inhibition of selectin expression.