论文部分内容阅读
[Objective]To establish a method for the determination of coixol in root,stem and testa of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf herbs by HPLC.[Method]HPLC was used and the conditions were as follows:Agilent HC-C18 column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% phosphoric acid(V:V=25:75),flow rate of 1.0 ml/min,column temperature of 25 ℃ and detection wavelength of 232 nm.[Result] The contents of coixol in different parts of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.were as follows:root>testa>stem.[Conclusion]This method is accurate and specific,which is useful for the integrative exploitation of coixol in root,testa and stem of Coix lacryma-jobi L.var.ma-yuen(Roman.)Stapf.
[Objective] To establish a method for the determination of coixol in root, stem and testa of Coix lacryma-jobi L. var. Ma-yuen (Roman.) Stapf herbs by HPLC. [Method] HPLC was used and the conditions were as The mobile phase of acetonitrile-0.1% phosphoric acid (V: V = 25:75), flow rate of 1.0 ml / min, column temperature of 25 ° C and detection wavelength of 232 nm. [Result] The contents of coixol in different parts of Coix lacryma-jobi L.var.ma-yuen (Roman.) Stapf.were as follows: root> testa> stem. [Conclusion] This method is accurate and specific, which is useful for the integrative exploitation of coixol in root, testa and stem of Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf.