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目的:测定抗坏血酸(AA)和DL-α-生育酚(αT)联合处理对人肝癌细胞增殖与再分化的作用。方法:维甲酸(RA)为阳性对照。用细胞表面电荷、生化变化和软琼脂细胞生长等指标测定细胞分化和增殖。结果:用AA 4 mmol·L~(-1)和αT 1 mmol·L~(-1)联合处理后,肝癌细胞的生长和分裂指数显著下降,增殖抑制率达61.3％。与恶化有关的指标显著减轻,如细胞表面电荷明显降低,电泳率从1.63 μm·s~(-1)·V~(-1)·cm~(-1)降低到0.89,甲胎蛋白由300μg·g~(-1)(protein)降到80,γ-谷氨酰转肽酶活性由0.81 U·g~(-1)(protein)降到0.201。与分化相关的酪氨酸-α-酮戊二酸转氨酶活性显著上升,由10.6μmol·g~(-1)(protein)升高到45,细胞克隆形成力降低96.6％。结论:AA和αT合用能够抑制人肝癌细胞增殖,诱导分化,并逆转恶性表型。
Objective: To determine the effect of combined treatment of ascorbic acid (AA) and DL-α-tocopherol (αT) on the proliferation and re-differentiation of human hepatoma cells. Methods: Retinoic acid (RA) as a positive control. Cell differentiation and proliferation were measured by cell surface charge, biochemical changes, and soft agar cell growth. Results: After treatment with AA 4 mmol·L -1 and αT 1 mmol·L -1, the growth and mitotic index of hepatocellular carcinoma cells were significantly decreased, and the inhibition rate of proliferation was 61.3%. The indicators related to exacerbations were significantly reduced. For example, the charge on the cell surface was significantly decreased, the electrophoresis rate decreased from 1.63 μm · s -1 · V -1 · cm -1 to 0.89, · G ~ (-1) decreased to 80, γ-glutamyl transpeptidase activity decreased from 0.81 U · g -1 protein to 0.201. The activity of tyrosine-α-ketoglutarate transaminase associated with differentiation increased significantly from 10.6μmol · g -1 to 45%, and the cell clonality decreased by 96.6%. Conclusion: Combination of AA and αT can inhibit the proliferation, induce differentiation and reverse the malignant phenotype of human hepatoma cells.