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目的:探讨急性局灶性脑梗死可塑性变化的星形胶质细胞在脑缺血损伤中的作用.方法:免疫组织化学和免疫荧光双标记技术. 结果:胶质原纤维酸性蛋白(GFAP)阳性星形胶质细胞在脑梗塞灶的周围发生肥大和增生性可塑改变,其突起的变化尤为显著. 粗大的突起呈纤维状,并相互交织呈密集的网,其末端向脑梗塞灶的中央延伸.在缺血周边的半影区可见谷氨酸转运体(EAAT1) 阳性表达,呈斑点和纤维状;共聚焦扫描显微镜下可见EAAT1与GFAP双标记的星形胶质细胞.结论:急性局灶性脑梗死后发生 可塑性变化的星形胶质细胞通过增强EAAT1的功能参与脑梗死的修复过程.
Objective: To investigate the role of plasticity-induced astrocytes in cerebral ischemic injury in acute focal cerebral infarction.Methods: Immunohistochemistry and immunofluorescence double labeling were used.Results: The glial fibrillary acidic protein (GFAP) positive Astrocytes appear hypertrophy and hyperplastic plastic changes around the cerebral infarction, the prominent changes of the astrocytes are particularly significant.The thick protrusions are fibrous, and intertwined with a dense network, the end of which to the central extension of cerebral infarction .The expression of glutamate transporter (EAAT1) in the penumbral area around the ischemic area was positive, with spots and fibrils.Under the confocal scanning microscope, EAAT1 and GFAP double-labeled astrocytes were observed.Conclusion: Astrocytes that undergo plastic changes after cerebral infarction are involved in the repair process of cerebral infarction by enhancing the function of EAAT1.