阿片样肽在人胎儿胸腺组织的表达

• 作 者: 李柏青 居弘 李涤臣 承泽农 章可仪
• 机 构: 蚌埠医学院,蚌埠医学院,蚌埠医学院,蚌埠医学院,蚌埠医学院
• 出 处: 蚌埠医学院学报
• 发表日期: 1999年05期
• 关键词: 胸腺组织 阿片样肽 胸腺上皮细胞 亮氨酸脑啡肽 内啡肽类 脑啡肽类 免疫组化检测 湿重 提取物 放射免疫测定

目的：探讨阿片样肽在人胎儿胸腺组织及其上皮细胞的表达。方法：用放射免疫测定药盒测定人胎儿胸腺组织匀浆提取物中β内啡肽（β Ｅ Ｐ） 和亮氨酸脑啡肽（ Ｌ Ｅ Ｎ Ｋ） ，同时用免疫组化方法检测胎儿胸腺组织冷冻切片和石蜡切片中的β Ｅ Ｐ 和 Ｌ Ｅ Ｎ Ｋ 的表达。结果：人胎儿胸腺组织提取液中β Ｅ Ｐ（１２ 例） 和 Ｌ Ｅ Ｎ Ｋ（１１ 例） 的含量（ｐｇ／１００ ｍｇ 湿重组织） 分别为５５０ ．８９±１９５ ．１１ 和１ ０２８ ．４４ ±６３２ ．７８ 。比较胎龄＜ ２０ 周组和≥２０ 周组的胸腺组织提取液中两种阿片样肽的含量差异均无显著性（ Ｐ＞ ０ ．０５） 。免疫组化检测发现，胸腺组织中β Ｅ Ｐ 和 Ｌ Ｅ Ｎ Ｋ 阳性反应细胞的分布部位与角蛋白阳性反应细胞相一致，表明胸腺上皮细胞可表达这两种阿片样肽。结论：本研究结果表明，人胎儿胸腺组织在胎龄１６ ～２０ 周时即可表达β Ｅ Ｐ 和 Ｌ Ｅ Ｎ Ｋ，胸腺上皮细胞与产生这两种阿片样肽有关。

Objective: To investigate the expression of opioid peptide in human fetal thymus and its epithelial cells. Methods: β-endorphin (β-EP) and leucine enkephalin (L-E N K) in human fetus thymus homogenate were determined by radioimmunoassay kit. Immunohistochemistry Detection of fetal thymus frozen sections and paraffin sections of β  E P and L  E N K expression. Results: The content of βEP (12 cases) and LE N K (11 cases) in human fetal thymus extract (pg / 100 mg wet weight tissue) were 550 and 550 respectively. 89 ± 195. 11 and 1 028. 44 ± 632. 78. There were no significant differences in the contents of two opioid peptides in thymus tissue between the gestational age groups <20 weeks and> 20 weeks (P> 0.05). The results of immunohistochemistry showed that the distribution of βEP and LENK positive cells in thymus was consistent with that of keratinpositive cells, suggesting that these two opioids could be expressed in thymus epithelial cells. Conclusion: The results of this study indicate that human fetal thymus can express βEP and LNN K at gestational age from 16 to 20 weeks. Thymus epithelial cells are involved in the production of these two opioid peptides.