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目的探讨烟酰胺对新生SD大鼠坏死性小肠结肠炎(NEC)模型肠道组织的保护作用。方法 3日龄SD大鼠64只随机分为4组,NEC模型+烟酰胺组、NEC模型组、生理盐水对照组、烟酰胺对照组每组16只。NEC模型通过缺氧复氧冷刺激7天制备,烟酰胺(20 mg/次)和生理盐水(5 ml/次)灌胃从实验7天后开始,每天1次,共7天。各组在实验第7、14天分别处死8只大鼠,解剖回盲部近端肠管行病理学检查,其他肠道组织制备组织匀浆检测肠组织血小板活化因子(PAF)水平。结果 NEC模型组实验中共死亡4只,其余组无死亡。NEC模型+烟酰胺组和NEC模型组第7天肠道组织病理评分[(2.56±0.70)分,(2.75±0.26)分]和PAF水平[(28 965±2 326)ng/ml,(22 570±1 156)ng/ml]均高于生理盐水对照组和烟酰胺对照组[(0.67±0.04)分,(0.90±0.02)分,(1 659±324)ng/ml,(1 446±226)ng/ml],差异有统计学意义(P<0.05),NEC模型+烟酰胺组和NEC模型组差异无统计学意义(P>0.05)。第14天NEC模型组肠道组织病理评分和PAF水平[(3.69±0.50)分,(28 965±1 023)ng/ml]均高于NEC模型+烟酰胺组[(1.55±0.34)分,(15 986±222)ng/ml],差异有统计学意义(P<0.05)。第7天和第14天肠组织病理损伤评分与肠组织PAF水平均成正相关(r=0.951、0.840,P<0.01)。结论烟酰胺可以减轻NEC大鼠模型肠道组织病理损伤、降低PAF水平,对NEC具有保护作用。
Objective To investigate the protective effect of nicotinamide on intestinal tissue of neonatal SD rats with necrotizing enterocolitis (NEC). Methods Sixty-four three-day-old SD rats were randomly divided into four groups. NEC model + nicotinamide group, NEC model group, saline control group and niacinamide control group were given 16 rats in each group. NEC models were prepared by cold stimulation with hypoxia and reoxygenation for 7 days. Nicotinamide (20 mg / time) and saline (5 ml / time) were orally administered starting 7 days after the experiment and once daily for 7 days. Eight rats were sacrificed on the 7th and 14th day of the experiment respectively. The proximal bowel of the ileocecal part was dissected for histopathological examination. Tissue homogenate was used to detect the platelet activating factor (PAF) in other intestinal tissues. Results A total of 4 deaths were observed in the NEC model group and none died in the remaining groups. The intestinal histopathological score [(2.56 ± 0.70) min, (2.75 ± 0.26) min] and PAF level [(28 965 ± 2 326) ng / ml, (22 570 ± 1 156 ng / ml] were significantly higher than those in saline control group and nicotinamide control group [(0.67 ± 0.04), (0.90 ± 0.02), (1665 ± 324) ng / ml, (1446 ± 226 ng / ml], the difference was statistically significant (P <0.05). There was no significant difference between NEC model + nicotinamide group and NEC model group (P> 0.05). On the 14th day, the intestinal histopathological score and PAF level in NEC model group were significantly higher than that in NEC model + nicotinamide group [(3.69 ± 0.50) vs (28 965 ± 1023) ng / ml [(1.55 ± 0.34) (15 986 ± 222) ng / ml], the difference was statistically significant (P <0.05). On the 7th and 14th day, pathological damage score of intestinal tissue was positively correlated with PAF level of intestinal tissue (r = 0.951,0.840, P <0.01). Conclusion Nicotinamide can reduce the pathological damage of intestinal tissue and decrease the level of PAF in NEC rats, which has a protective effect on NEC.