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2014年在广东辣椒产区发现疑似番茄斑萎病毒属(Tospovirus)病毒侵染引起的辣椒褪绿坏死环斑症状。Dot-ELISA检测显示,该病毒分离物与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)单克隆抗体不产生血清学反应,表明该病毒不属于TSWV。利用Tospovirus属病毒通用引物gL3637/gL4435C进行RT-PCR检测,可以从所有辣椒病样总RNA中扩增出预期大小约800 bp的目的片段。基因克隆与序列分析表明,该片段序列与西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)中国广州分离物的同源性最高,为97.5%。系统进化分析也显示,该病毒分离物与WSMoV各分离物亲缘关系最近,并聚类在一个分支。因此,侵染广东辣椒的病毒分离物为WSMoV。
In 2014, it was discovered in the chilli pepper producing area of Guangdong that the chlorotic chlorotic necrosis of the annulus annulus caused by the infection of the tomato tomatopotvirus (Tospovirus) is suspected. The results of Dot-ELISA showed that there was no serological reaction between the isolate and the monoclonal antibody against Tomato spotted wilt virus (TSWV), indicating that the virus did not belong to TSWV. RT-PCR detection of the common virus of Tospovirus genus gL3637 / gL4435C can amplify the expected fragment of about 800 bp from all pepper-like total RNA samples. The cloning and sequence analysis showed that the fragment had the highest homology (97.5%) with the isolate of watermelon silver mottle virus (WSMoV) from Guangzhou, China. Phylogenetic analysis also showed that the virus isolate was closest to WSMoV isolates and clustered in one branch. Therefore, the virus isolate that infected Guangdong pepper was WSMoV.