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目的体外观察葡萄糖对间充质干细胞(BMSCs)向成骨细胞(OB)分化的影响,探讨其对骨代谢的作用机制。方法从大鼠长骨骨髓分离获取间充质干细胞,在不同糖浓度(5.6mmol/l、25mmol/l、50mmol/l)干预下向成骨细胞诱导分化培养21d;茜素红染色、光镜计数矿化率;realtime PCR测定OB的标记物碱性磷酸酶(ALP),骨钙素(BGP)及转录因子Runx2 mRNA表达,进行不同糖浓度组干预后的变化比较。结果显示随着糖浓度升高,矿化率显著降低,差异有统计学意义(P<0.05)。与5.6mmol/l组比较,50mmol/l组的ALP,BGP及Runx2 mRNA表达减少,分别为73%(P<0.05)、69%(P<0.05)及70%(P<0.05),25mmol/l组的ALP、BGP及Runx2 mRNA表达下降也具有显著性(P<0.05)。结论高糖抑制BMSCs向OB分化,可能为糖尿病性骨质疏松形成的重要机制之一。
Objective To observe the effect of glucose on the differentiation of mesenchymal stem cells (BMSCs) into osteoblasts (OB) in vitro and to explore its mechanism of action on bone metabolism. Methods Mesenchymal stem cells (MSCs) were isolated from the long bone marrow of rats and differentiated into osteoblasts under different concentrations of glucose (5.6mmol / l, 25mmol / l, 50mmol / l) for 21 days. Alizarin red staining, Mineralization rate; ALT, BGP and Runx2 mRNA expression of OB were measured by realtime PCR, and compared with the intervention of different sugar concentration group. The results showed that with the increase of sugar concentration, the mineralization rate was significantly reduced, the difference was statistically significant (P <0.05). Compared with 5.6mmol / l group, the expression of ALP, BGP and Runx2 mRNA in 50mmol / l group decreased by 73% (P <0.05), 69% (P <0.05) and 70% l group ALP, BGP and Runx2 mRNA expression decreased also significant (P <0.05). Conclusion High glucose inhibits the differentiation of BMSCs into OB, which may be one of the important mechanisms of the formation of diabetic osteoporosis.