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目的:通过观察消炎长皮膏对大鼠创面修复的影响,从分子生物学水平探讨其作用机制,为其临床应用提供理论基础。方法:将大鼠背部造成创面感染坏死模型。将大鼠随机分为2组,消炎长皮膏与凡士林组,于第7天、第14天记录创面面积、伤腔容积,并取创面组织行组织学观察及流式细胞学检测,免疫组化检测创面Ⅰ型胶、Ⅲ型胶、FGF2表达,ELISA检测创面及血浆FN表达。结果:消炎长皮膏组各时间点创面面积和伤腔容积均明显缩小(P<0.05),消炎长皮膏组治疗7 d后创面组织中Ⅰ型胶、Ⅲ型胶、FGF2表达及FN表达明显高于对照组,两组比较差异有统计学意义(P<0.05)。S期细胞百分比明显高于对照组(P<0.05)。组织学检查发现消炎长皮膏组成纤维细胞活跃,毛细血管丰富。结论:消炎长皮膏可促进创面愈合,同时减少瘢痕形成。
OBJECTIVE: To observe the effect of anti-inflammatory long-skin cream on wound healing in rats and to explore its mechanism from the molecular biology level to provide a theoretical basis for its clinical application. Methods: The wounds on the back of rats were induced necrosis model. The rats were randomly divided into 2 groups: Anti-inflammatory Changpi Pill and Vaseline Group. On the 7th day and the 14th day, the area of wounds and the volume of wounds were recorded. The wound tissues were harvested for histological observation and flow cytometry. The expressions of type I glue, type III glue and FGF2 in wounds were detected, and the expression of FN in wounds and plasma was detected by ELISA. Results: The area of wounds and volume of injured wounds were significantly reduced in the anti-inflammatory Changpi group at each time point (P <0.05). The expression of type I collagen, type Ⅲ collagen, FGF2 and FN in wounds treated with anti-inflammatory Changpi group for 7 days Significantly higher than the control group, the difference between the two groups was statistically significant (P <0.05). S phase cells was significantly higher than the control group (P <0.05). Histological examination found that anti-inflammatory Changpi composition of fibroblasts, capillary rich. Conclusion: Anti-inflammatory long-skin plaster can promote wound healing and reduce scar formation.