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背景:肾缺血/再灌注诱导产生大量活性氧,导致核因子κB的活化。激活的核因子κB通过调节诱导型一氧化氮合酶的生成,进而导致一氧化氮的大量产生和触发细胞凋亡。目的:观察吡咯烷二硫代氨基甲酸对肾缺血再灌注后肾脏组织中核因子κB、诱导型一氧化氮合酶、一氧化氮、caspase-3和细胞凋亡指数的作用。方法:将健康雄性 Wistar 大鼠随机分为 3组:缺血再灌注组和吡咯烷二硫代氨基甲酸组通过右侧肾切除+左肾动脉夹闭45 min 建立肾缺血/再灌注模型,吡咯烷二硫代氨基甲酸组于实验前 30 min 尾静脉注射吡咯烷二硫代氨基甲酸(100 mg/kg)。假手术组不给予缺血再灌注处理。结果与结论:与假手术组相比,缺血再灌注组大鼠再灌注后肾组织核因子κB表达水平、血肌酐水平、尿素氮水平、诱导型一氧化氮合酶活性、一氧化氮表达水平、caspase-3 表达水平和细胞凋亡水平增加(P<0.05);而与缺血再灌注组相比,吡咯烷二硫代氨基甲酸组大鼠再灌注后以上指标均好转。说明肾缺血/再灌注损伤可引起肾组织结构损伤和细胞凋亡,且与核因子κB引起的一氧化氮高表达有关;应用核因子κB抑制剂吡咯烷二硫代氨基甲酸可对缺血再灌注肾损伤发挥明显的保护作用。
BACKGROUND: Renal ischemia / reperfusion induces production of large quantities of reactive oxygen species leading to the activation of nuclear factor κB. Activated nuclear factor kappa B regulates the production of inducible nitric oxide synthase, leading to the production of nitric oxide in large quantities and triggering apoptosis. Objective: To observe the effects of pyrrolidine dithiocarbamic acid on the expression of NF-kappaB, nitric oxide, caspase-3 and apoptosis in renal tissues after renal ischemia-reperfusion injury. Methods: Healthy male Wistar rats were randomly divided into 3 groups: Ischemia-reperfusion group and pyrrolidine dithiocarbamate group, renal ischemia / reperfusion model was established by right nephrectomy + left renal artery occlusion for 45 min, Pyrrolidine dithiocarbamate group was injected intravenously with pyrrolidine dithiocarbamic acid (100 mg / kg) 30 min before the experiment. Sham-operated group was not given ischemia-reperfusion treatment. RESULTS AND CONCLUSION: Compared with the sham-operation group, the expression of NF-κB, serum creatinine, urea nitrogen, inducible nitric oxide synthase and nitric oxide The level of caspase-3 and the level of apoptosis increased (P <0.05). Compared with the ischemia-reperfusion group, the above indexes of pyrrolidine dithiocarbamate group were all improved after reperfusion. Renal ischemia / reperfusion injury can cause structural damage and apoptosis in renal tissues, and is related to the high expression of nitric oxide induced by NF-κB. Pyrrolidine dithiocarbamate, an inhibitor of NF-κB, Reperfusion renal injury play a significant protective effect.