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目的:探讨人类白细胞抗原HLA-DRB1基因多态性与南疆维吾尔族宫颈癌的关系。方法:采用聚合酶联反应序列特异性寡核苷酸探针(Polymerase chain reaction sequence-specific oligonucleotide,PCR-SSO)法对200例南疆维吾尔族宫颈癌患者及200例正常妇女宫颈组织中检测HLA-DRB1的等位基因。结果:1)HLA-DRB1*15在宫颈癌组中出现的频率明显高于对照组,并有统计学差异,OR>1(χ2=10.890,P=0.001,OR=2.061)。2)HLA-DRB1*08在宫颈癌组中出现的频率明显低于对照组,并有统计学差异,OR<1(χ2=4.061,P=0.044,OR=0.463)。3)HLA-DRB1的其他等位基因在宫颈癌组及对照组中出现的频率无显著性差异(P>0.05)。结论:HLA-DRB1*15可能与维吾尔族妇女对宫颈癌的遗传易感性有关,是维吾尔族妇女对宫颈癌的易感基因;可能是维吾尔族妇女宫颈癌患病率高的原因之一。而HLA-DRB1*08可能为维吾尔族妇女宫颈癌的保护基因,维吾尔族妇女宫颈癌易感基因及保护基因的检测可能在高危人群的检测及群体遗传干预工作中有一定的指导意义。
Objective: To investigate the relationship between human leukocyte antigen HLA-DRB1 gene polymorphism and Uygur cervical cancer in southern Xinjiang. Methods: 200 cases of cervical cancer from Uygur nationality in South Xinjiang and 200 cases of normal women were detected by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) -DRB1 allele. Results: 1) The frequency of HLA-DRB1 * 15 in cervical cancer group was significantly higher than that in control group (OR> 1, χ2 = 10.890, P = 0.001, OR = 2.061). 2) The frequency of HLA-DRB1 * 08 in cervical cancer group was significantly lower than that in control group (OR <1, χ2 = 4.061, P = 0.044, OR = 0.463). 3) There was no significant difference in the frequencies of other alleles of HLA-DRB1 between cervical cancer group and control group (P> 0.05). Conclusion: HLA-DRB1 * 15 may be related to the genetic susceptibility of Uighur women to cervical cancer and is a susceptible gene to cervical cancer among Uighur women. It may be one of the reasons for the high prevalence of cervical cancer in Uighur women. HLA-DRB1 * 08 may be the protective gene for cervical cancer in Uighur women. The detection of cervical cancer susceptibility genes and protective genes in Uyghur women may have some guiding significance in the detection of high-risk groups and genetic intervention in population.