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本文报告了用气相色谱法测定大鼠肝细胞色素Ｐ４５０２Ｅ１的酶活性。在一定的实验条件下，使丁醇在微粒体中被氧化为丁醛，用顶空气相色谱法测定产生的丁醛量，并测定肝微粒体中的蛋白质及细胞色素Ｐ４５０总量，可算出细胞色素Ｐ４５０催化丁醇氧化的速率，实验表明，乙醇诱导的细胞色素Ｐ４５０２Ｅ１明显增大了丁醇氧化的速率，而其他典型诱导剂诱导的细胞色素Ｐ４５０异构酶不增大丁醇氧化速率，所以，细胞色素Ｐ４５０催化丁醇氧化的速率可作为判断细胞色素Ｐ４５０２Ｅ１酶活性的指标。
This article reports the determination of the enzymatic activity of rat liver cytochrome P4502E1 by gas chromatography. Under certain experimental conditions, butanol is oxidized to butanal in the microsome, the amount of butyraldehyde produced is determined by headspace gas chromatography, and the total amount of protein and cytochrome P450 in the liver microsome is determined Cytochrome P450 catalyzes the rate of butanol oxidation. Experiments show that ethanol-induced cytochrome P450 2E1 significantly increases the rate of butanol oxidation, while other typical inducers induce cytochrome P450 isomerase does not increase butanol oxidation rate, Therefore, the rate of cytochrome P450 catalyzed butanol oxidation can be used as an indicator of cytochrome P450 E2E activity.