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目的:观察野生剌梨超氧化物歧化酶(SOD)提取液对大鼠慢性四氯化碳肝损伤的保护和治疗作用。方法:36只雄性Wistar大鼠皮下注射四氯化碳制造慢性肝损伤模型。预防组9只在造模同时给予刺梨SOD提取液灌胃,6周后处死 治疗组9只在造模停止后同样给药6周后处死。相应设立未预防对照组9只和未治疗对照组9只作比较研究。结果:预防组血清ALT明显低于未预防组(P<0.05):治疗组血清AST明显低于未治疗组(P<0.05)。肝组织匀浆脂质过氧化指标中预防组MDA水平明显低于未预防组(P<0.05),GBH-Px水平明显高于未预防组:治疗组GSH-Px水平明显高于未治疗组(P<0.05)。肝组织病理学观察证明预防组HAI积分明显低于未预防组(P<0.05)。结论:口服植物源性野生刺梨SOD制剂作为抗氧化剂对大鼠慢性四氯化碳肝损伤有一定预防保护和治疗作用。
Objective: To observe the protective and therapeutic effects of wild assassin pear superoxide dismutase (SOD) on chronic carbon tetrachloride-induced liver injury in rats. Methods: Thirty - six male Wistar rats were injected subcutaneously with carbon tetrachloride to make chronic liver injury model. Nine rats in the preventive group were given intragastric administration of SOD extract of Rosa roxburghii at the same time. After 6 weeks, 9 rats in the treatment group were also sacrificed 6 weeks after the model was stopped. Nine non-prophylaxis controls and nine untreated controls were compared. Results: Serum ALT levels in the prevention group were significantly lower than those in the non-prevention group (P <0.05). Serum AST in the treatment group was significantly lower than that in the untreated group (P <0.05). The levels of MDA in the liver homogenate lipid peroxidation prevention group were significantly lower than those in the non-prevention group (P <0.05), and the levels of GBH-Px in the prevention group were significantly higher than those in the non-prevention group. The GSH-Px level in the treatment group was significantly higher than that in the untreated group P <0.05). Liver histopathology showed HAI scores in prevention group were significantly lower than those in non-prevention group (P <0.05). CONCLUSION: Oral administration of SOD from wild prickly pear could prevent and treat chronic liver injury caused by CCl 4 as an antioxidant.