正常-脑瘫双生子基因表达谱差异研究

• 作 者: 张天娥 董小丽 王米渠
• 机 构: 成都中医药大学,成都市第一人民医院,
• 出 处: 辽宁中医杂志
• 发表日期: 2009年11期
• 关键词: 基因表达谱 脑瘫 特异性状 差异研究 信息挖掘 差异表达 下调基因 基因组 小儿脑瘫 姿势异常

目的:在前期脑瘫双生子病证结合研究的基础上,进一步进行了基因表达谱的研究,整体把握脑瘫患儿与正常儿之间存在的微观差异,以加深对该病证认识,为提供新的治疗途径与诊断方法打下基础。方法:人22000点的全基因组表达谱芯片检测与信息挖掘。结果:获得差异表达基因2703条,其中1272条上调,1431条基因下调。结论:对某一特异性状或表型而言,采用双生子作为研究对象,利用基因芯片的方法可高效的涌现代表该病证特征的差异基因。

OBJECTIVE: To further study the gene expression profiling based on the combined study of twins syndrome of cerebral palsy, and to grasp the microscopic differences between children with cerebral palsy and normal children in order to deepen the understanding of the disease syndrome and to provide new The treatment and diagnosis methods to lay the foundation. Methods: Whole genome cDNA microarray detection and information mining of 22000 human. Results: 2703 differentially expressed genes were obtained, of which 1272 were up-regulated and 1431 genes were down-regulated. Conclusion: For a specific trait or phenotype, using twins as the research object, gene chips can be used to efficiently generate the differentially expressed genes that are characteristic of the disease.